Domański Leszek, Dołgowska Barbara, Safranow Krzysztof, Myślak Marek, Rózański Jacek, Romanowski Maciej, Ostrowski Marek, Domański Maciej, Pawlik Andrzej, Ciechanowski Kazimierz
Department of Nephrology, Transplantology and Internal Medicine, Pomeranian Medical University, Szczeci, Poland.
Nephrology (Carlton). 2006 Oct;11(5):467-70. doi: 10.1111/j.1440-1797.2006.00672.x.
Generation of reactive oxygen specimens is the basic mechanism leading to ischaemia/reperfusion injury of the kidney graft. Oxygen burst is a trigger for sophisticated biochemical changes leading to generation of oxygenated lipids and changes in microcirculation, which recruit recipient's neutrophils and contribute to delayed graft function. It has been shown that the free radicals generation correlates with the activity of anti-oxidative system. Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione (GSH) are involved in protection against free radicals.
To examine the activity of erythrocyte anti-oxidative system during reperfusion of the transplanted kidney allograft.
The study included 40 renal transplant recipients. Blood was taken from the iliac vein before transplantation and from the graft's renal vein immediately, as well as 2 and 4 min after total reperfusion. The authors assessed the process of reperfusion using ThermaCAM SC500 termovision camera. Spectrophotometric methods were used to measure superoxide dismutase, glutathione peroxidase and catalase activity as well as glutathione concentrations in erythrocytes.
There were no statistically significant differences in the activities of superoxide dismutase, catalase and glutathione peroxidase as well as glutathione concentrations during the first 4 min after total graft reperfusion. Nevertheless, there was a positive correlation between the activity of superoxide dismutase and glutathione peroxidase.
The results suggest that the erythrocyte anti-oxidative system is stable during the early phase after reperfusion. An association between some anti-oxidative enzymes was noted.
活性氧标本的产生是导致肾移植缺血/再灌注损伤的基本机制。氧爆发是引发复杂生化变化的诱因，这些变化会导致氧化脂质的产生和微循环的改变，进而招募受体的中性粒细胞并导致移植肾功能延迟。研究表明，自由基的产生与抗氧化系统的活性相关。超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）和谷胱甘肽（GSH）参与自由基的防御。
研究移植肾同种异体移植物再灌注期间红细胞抗氧化系统的活性。
该研究纳入40例肾移植受者。在移植前从髂静脉采血，在完全再灌注即刻以及再灌注2分钟和4分钟后从移植肾静脉采血。作者使用ThermaCAM SC500热成像相机评估再灌注过程。采用分光光度法测量红细胞中超氧化物歧化酶、谷胱甘肽过氧化物酶和过氧化氢酶的活性以及谷胱甘肽浓度。
在移植肾完全再灌注后的前4分钟内，超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的活性以及谷胱甘肽浓度无统计学显著差异。然而，超氧化物歧化酶和谷胱甘肽过氧化物酶的活性之间存在正相关。
结果表明，再灌注后早期红细胞抗氧化系统稳定。注意到一些抗氧化酶之间存在关联。
