一种高分辨率钨酸盐膜标记物。

A high-resolution tungstate membrane label.

作者信息

Hainfeld J F, Lipka J J, Quaite F E

机构信息

Biology Department, Brookhaven National Labortory, Upton, New York 11973.

出版信息

J Histochem Cytochem. 1990 Dec;38(12):1795-803. doi: 10.1177/38.12.1701459.

DOI:10.1177/38.12.1701459

PMID:1701459

Abstract

A new class of membrane labels was synthesized which contain a tungstate cluster (having 11 tungsten atoms) and an aliphatic organo-tin moiety with various chain lengths (C4, C8, C12, C18, C22). These molecules were found to insert into synthetic phospholipid vesicles and biological membranes (human red blood cell membranes). The tungstate clusters can be individually visualized in the high resolution STEM or seen en mass in thin-sectioned labeled membranes in the CTEM. These new labels should provide a means for direct high-resolution imaging of lipid-phase systems.

摘要

合成了一类新型的膜标记物，其包含一个钨酸盐簇（含有11个钨原子）和一个具有不同链长（C4、C8、C12、C18、C22）的脂肪族有机锡部分。发现这些分子可插入合成磷脂囊泡和生物膜（人红细胞膜）中。钨酸盐簇在高分辨率扫描透射电子显微镜（STEM）中可单独可视化，或在扫描透射电子显微镜（CTEM）中薄切片标记膜中整体可见。这些新的标记物应为脂质相系统的直接高分辨率成像提供一种手段。