Wen Hui-Ju, Lin Ying-Chu, Lee Yung-Ling, Guo Yueliang Leon
Department of Environmental and Occupational Health, National Cheng Kung University Medical College, Tainan, Taiwan.
Pediatr Allergy Immunol. 2006 Nov;17(7):489-94. doi: 10.1111/j.1399-3038.2006.00461.x.
High cord blood immunoglobulin E (cbIgE) is known to be associated with increased risks of atopic diseases in childhood. The relationship between genetic polymorphisms and high cbIgE has not been well documented. A cross-sectional study was conducted to assess the association between cbIgE and genetic polymorphisms of interleukin (IL)-4 -590C/T, the beta-subunit of the high-affinity receptor for IgE (FcepsilonRI-beta) E237G, lymphotoxin (LT)-alphaNcoI alleles, and tumor necrosis factor (TNF)-alpha -308G/A. A total of 320 mother-neonate pairs were recruited from four maternity hospitals from different locations of Taiwan. Cord blood was obtained and assayed for cbIgE. Polymerase chain reaction followed by restriction fragment length polymorphism was used to assess the genotypes. Three hundred pairs of mothers and neonates were included in the final analysis. Infants with IL-4 -590 C allele were found to have higher risk of elevated cbIgE (> or =0.35 IU/ml, 24.3%) (p = 0.004). After adjusting for gender, birth order, maternal age, and history of allergic disease in maternal and paternal families, odds ratios for CC and CT genotypes were 4.41 and 3.16 (95% confidence interval 0.78-22.67, and 1.66-6.13), respectively, using TT genotype as reference. The genotypes of FcepsilonRI-beta, LT-alpha, and TNF-alpha were not associated with cbIgE before or after the adjustment. Our finding suggested a significant association of cbIgE with genetic polymorphism of IL-4 -590C/T, but not with the genotypes of FcepsilonRI-beta, LT-alpha, and TNF-alpha.
已知高脐带血免疫球蛋白E（cbIgE）与儿童期特应性疾病风险增加相关。基因多态性与高cbIgE之间的关系尚未得到充分记录。进行了一项横断面研究，以评估cbIgE与白细胞介素（IL）-4 -590C/T、IgE高亲和力受体β亚基（FcepsilonRI-β）E237G、淋巴毒素（LT）-α NcoI等位基因以及肿瘤坏死因子（TNF）-α -308G/A基因多态性之间的关联。从台湾不同地区的四家妇产医院招募了总共320对母婴对。采集脐带血并检测cbIgE。采用聚合酶链反应后接限制性片段长度多态性方法评估基因型。最终分析纳入了300对母婴。发现携带IL-4 -590 C等位基因的婴儿发生cbIgE升高（≥0.35 IU/ml，24.3%）的风险更高（p = 0.004）。在调整了性别、出生顺序、母亲年龄以及母亲和父亲家族中的过敏性疾病史后，以TT基因型为参照，CC和CT基因型的比值比分别为4.41和3.16（95%置信区间分别为0.78 - 22.67和1.66 - 6.13）。FcepsilonRI-β、LT-α和TNF-α的基因型在调整前后均与cbIgE无关。我们的研究结果表明，cbIgE与IL-4 -590C/T基因多态性显著相关，但与FcepsilonRI-β、LT-α和TNF-α的基因型无关。
