Xun S, Jingdong T, Zhaonan Z, Xinyuan L
Institute of Biophysics, Chinese Academy of Sciences, Beijing 100080, P.R. China.
Biophys Chem. 1991 May;40(2):161-7. doi: 10.1016/0301-4622(91)87005-p.
Linoleic acid was used as a model system to study lipid peroxidation initiated by the reaction of ferrous iron with hydrogen peroxide. Low-level chemiluminescence of the peroxidation was measured with a high-sensitivity single-photon counter. It was found that the luminescence primarily comes from the dimol reaction of singlet oxygen and that the peak intensity of emission is a quadratic function of the concentration of either Fe2+ or H2O2, provided that the other Fenton reagent is in great excess. Under the same conditions, analysis on reaction kinetics shows a linear relationship between the maximal level of the initiator formed by the Fenton reaction and the initial concentration of Fe2+ or H2O2. This implies that the peak intensity of the chemiluminescence may be a good index of the maximal level of the initiator.
以亚油酸作为模型体系，研究亚铁离子与过氧化氢反应引发的脂质过氧化作用。用过高灵敏度单光子计数器测量过氧化作用的低水平化学发光。结果发现，发光主要来自单线态氧的双分子反应，并且只要另一种芬顿试剂过量，发射峰强度就是Fe2+或H2O2浓度的二次函数。在相同条件下，反应动力学分析表明，芬顿反应形成的引发剂的最大水平与Fe2+或H2O2的初始浓度之间呈线性关系。这意味着化学发光的峰强度可能是引发剂最大水平的良好指标。
