Hanson Kerry M, Gratton Enrico, Bardeen Christopher J
Department of Chemistry, University of California at Riverside, Riverside, CA 92506, USA.
Free Radic Biol Med. 2006 Oct 15;41(8):1205-12. doi: 10.1016/j.freeradbiomed.2006.06.011. Epub 2006 Jul 6.
The number of UV-induced (20 mJ cm(-2)) reactive oxygen species (ROS) generated in nucleated epidermis is dependent upon the length of time the UV filter octocrylene, octylmethoxycinnamate, or benzophenone-3 remains on the skin surface. Two-photon fluorescence images acquired immediately after application of each formulation (2 mg cm(-2)) to the skin surface show that the number of ROS produced is dramatically reduced relative to the skin-UV filter control. After each UV filter remains on the skin surface for t=20 min, the number of ROS generated increases, although it remains below the number generated in the control. By t=60 min, the filters generate ROS above the control. The data show that when all three of the UV filters penetrate into the nucleated layers, the level of ROS increases above that produced naturally by epidermal chromophores under UV illumination.
紫外线诱导（20 mJ/cm²）产生于有核表皮中的活性氧（ROS）数量，取决于紫外线过滤剂二乙氨羟苯甲酰基苯甲酸己酯、桂皮酸盐或二苯甲酮-3停留在皮肤表面的时间长度。在将每种配方（2 mg/cm²）涂抹于皮肤表面后立即采集的双光子荧光图像显示，相对于皮肤-紫外线过滤剂对照组，产生的ROS数量显著减少。在每种紫外线过滤剂停留在皮肤表面t = 20分钟后，产生的ROS数量增加，尽管仍低于对照组产生的数量。到t = 60分钟时，这些过滤剂产生的ROS高于对照组。数据表明，当所有三种紫外线过滤剂都渗透到有核层时，ROS水平会高于紫外线照射下表皮发色团自然产生的水平。
