Dudal Sherri, Turriere Chrystell, Bessoles Stephanie, Fontes Pascaline, Sanchez Françoise, Liautard Janny, Liautard Jean-Pierre, Lafont Virginie
Institut National de la Santé et de la Recherche Médicale, Unité 431, Université Montpellier 2, Montpellier, France.
J Immunol. 2006 Oct 15;177(8):5533-9. doi: 10.4049/jimmunol.177.8.5533.
Human Vgamma9Vdelta2 T cells play a crucial role in early immune response to intracellular pathogens. Moreover, in brucellosis, these cells are drastically increased in the peripheral blood of patients during the acute phase of infection. In vitro, Vgamma9Vdelta2 T cells are capable of inhibiting Brucella growth and development through a combination of mechanisms: 1) cytotoxicity, 2) macrophage activation and bactericidal activity through cytokine and chemokine secretion, and 3) antibacterial effects. We previously described that antibacterial factors were found in supernatants from activated Vgamma9Vdelta2 T cells. In this study, we show that Vgamma9Vdelta2 T cells express the human cathelicidin hCAP18 and its mature form, known as LL-37, is released upon activation of Vgamma9Vdelta2 T cells. We also show that LL-37 has an antibacterial effect on Brucella suis. Overall, our results demonstrate that LL-37 is a soluble factor responsible for a part of the bactericidal activity of Vgamma9Vdelta2 T cells.
人Vγ9Vδ2 T细胞在对细胞内病原体的早期免疫反应中起关键作用。此外，在布鲁氏菌病中，在感染急性期患者的外周血中这些细胞显著增加。在体外，Vγ9Vδ2 T细胞能够通过多种机制抑制布鲁氏菌的生长和发育：1）细胞毒性，2）通过细胞因子和趋化因子分泌激活巨噬细胞和杀菌活性，以及3）抗菌作用。我们之前描述过在活化的Vγ9Vδ2 T细胞的上清液中发现了抗菌因子。在本研究中，我们表明Vγ9Vδ2 T细胞表达人cathelicidin hCAP18，其成熟形式即LL-37在Vγ9Vδ2 T细胞活化时释放。我们还表明LL-37对猪布鲁氏菌有抗菌作用。总体而言，我们的结果证明LL-37是一种可溶性因子，负责Vγ9Vδ2 T细胞部分杀菌活性。
