Peng Fangyu, Lu Xin, Janisse James, Muzik Otto, Shields Anthony F
Department of Pediatrics, School of Medicine, Wayne State University, Detroit, Michigan 48201, USA.
J Nucl Med. 2006 Oct;47(10):1649-52.
Our objective was to determine whether human prostate cancer xenografts in mice can be localized by PET using 64CuCl2 as a probe (64Cu PET).
Athymic mice bearing human prostate cancer xenografts were subjected to 64Cu PET, followed by quantitative analysis of the tracer concentrations and immunohistochemistry study of human copper transporter 1 expression in the tumor tissues.
Human prostate cancer xenografts expressing high levels of human copper transporter 1 were well visualized on the PET images obtained 24 h after injection but not on the images obtained 1 h after injection. PET quantitative analysis demonstrated a high concentration of 64CuCl2 in the tumors in comparison to that in the left shoulder regions (percentage injected dose per gram of tissue: 3.6 +/- 1.3 and 0.6 +/- 0.3, respectively; P = 0.004), at 24 h after injection.
The data from this study suggested that locally recurrent prostate cancer might be localized with 64Cu PET using 64CuCl2 as a probe.
我们的目的是确定使用64CuCl2作为探针的PET（64Cu PET）是否能够定位小鼠体内的人前列腺癌异种移植瘤。
对携带人前列腺癌异种移植瘤的无胸腺小鼠进行64Cu PET检查，随后对示踪剂浓度进行定量分析，并对肿瘤组织中人铜转运蛋白1的表达进行免疫组织化学研究。
在注射后24小时获得的PET图像上，高表达人铜转运蛋白1的人前列腺癌异种移植瘤清晰可见，但在注射后1小时获得的图像上则不可见。PET定量分析表明，与左肩部区域相比，注射后24小时肿瘤内64CuCl2浓度较高（每克组织注射剂量百分比：分别为3.6±1.3和0.6±0.3；P = 0.004）。
本研究数据表明，使用64CuCl2作为探针的64Cu PET可能定位局部复发性前列腺癌。
