Ventura Meritxell, Guillén David, Anaya Ivan, Broto-Puig Francesc, Lliberia Josep Lluís, Agut Montserrat, Comellas Lluís
Analytical Chemistry Department, Institut Químic de Sarrià (Ramon Llull University), Via Augusta 390, 08017 Barcelona, Spain.
Rapid Commun Mass Spectrom. 2006;20(21):3199-204. doi: 10.1002/rcm.2723.
The simultaneous analysis of aflatoxins B1, G1, B2, G2 and ochratoxin A in beer was carried out by ultra-performance liquid chromatography/tandem mass spectrometry (UPLC/MS/MS). Mycotoxins were extracted, purified and concentrated from the beer sample in one step using a solid-phase extraction (SPE) cartridge that contained a polymeric sorbent. Optimization of different parameters, such as type of SPE sorbent, type and amount of wash solvent and pH of the sample, was carried out. The mobile phase consisted of a gradient of methanol + water (0.1% HCOOH) and a reversed-phase C18 column was used for the separation. The mass spectrometer used an electrospray ionization source operated in the positive mode to detect aflatoxins and in the negative mode to detect ochratoxin. UPLC/MS/MS is a rapid and sensitive technique that allows the separation of the five toxins in only 3.2 min. The limit of detection is 1 pg.
采用超高效液相色谱/串联质谱法（UPLC/MS/MS）对啤酒中的黄曲霉毒素B1、G1、B2、G2和赭曲霉毒素A进行同步分析。使用含有聚合物吸附剂的固相萃取（SPE）柱，一步从啤酒样品中提取、纯化和浓缩霉菌毒素。对不同参数进行了优化，如SPE吸附剂类型、洗涤溶剂类型和用量以及样品pH值。流动相由甲醇+水（0.1%甲酸）梯度组成，采用反相C18柱进行分离。质谱仪使用电喷雾电离源，以正模式检测黄曲霉毒素，以负模式检测赭曲霉毒素。UPLC/MS/MS是一种快速灵敏的技术，仅需3.2分钟就能分离出这五种毒素。检测限为1皮克。
