Zhao Sheng-Ping, Zhang Dan, Tan Li-Hong, Yu Bao, Cao Wei-Guo
College of Traditional Chinese Medicine, Chongqing Medical University, 400016, Chongqing, People's Republic of China.
The Lab of Traditional Chinese Medicine, Chongqing Medical University, 400016, Chongqing, People's Republic of China.
Sci Rep. 2016 Aug 4;6:30822. doi: 10.1038/srep30822.
A classification system for analytical methods was developed for the first time to determine the presence of aflatoxins B1, B2, G1 and G2 in traditional Chinese medicines (TCMs) based on different matrix types using ultra-performance liquid chromatography-tandem mass spectrometry. A useful characteristic of the approach was that the TCMs could be systematically divided into four categories (i.e., volatile oils, proteins, polysaccharides and fatty oils) depending on the matrix types. The approach concluded that different types of TCMs required different optimal sample preparation procedures. Based on the optimized analytical conditions, the limits of detection and quantification, average recoveries and linearity of four aflatoxins were determined and conformed to research limits. Of 22 TCMs samples, 14 samples were contaminated with at least one type aflatoxin at concentrations ranging from 0.2 to 7.5 μg/kg, and the average contents of aflatoxins were significantly different for the different matrix types. Moreover, we found a potential link between the contamination levels of aflatoxins and matrix types. TCMs containing fatty oils were the most susceptible to contamination by aflatoxins and followed by TCMs containing polysaccharides and proteins; TCMs containing abundant amounts of volatile oils were less prone to contamination.
首次开发了一种分析方法分类系统，用于基于不同基质类型，采用超高效液相色谱-串联质谱法测定中药中黄曲霉毒素B1、B2、G1和G2的存在情况。该方法的一个有用特性是，根据基质类型，中药可系统地分为四类（即挥发油、蛋白质、多糖和脂肪油）。该方法得出结论，不同类型的中药需要不同的最佳样品制备程序。基于优化的分析条件，测定了四种黄曲霉毒素的检测限、定量限、平均回收率和线性，并符合研究限值。在22份中药样品中，有14份样品被至少一种黄曲霉毒素污染，浓度范围为0.2至7.5μg/kg，不同基质类型的黄曲霉毒素平均含量存在显著差异。此外，我们发现了黄曲霉毒素污染水平与基质类型之间的潜在联系。含脂肪油的中药最易受到黄曲霉毒素污染，其次是含多糖和蛋白质的中药；含大量挥发油的中药较不易受到污染。
