Yang Xuedong, Deng Zhicheng, Wang Jing, Ding Mingyu
College of Pharmaceuticals and Biotechnology, Tianjin University, Tianjin 300072, China.
Se Pu. 2006 Jul;24(4):363-6.
A method was established for the isolation of soybean isoflavone glucosides from the total isoflavone extracts of soybean using preparative reversed-phase high performance liquid chromatography (RP-HPLC). The total isoflavone extracts were separated into four parts by solvent extraction, those are the ethyl acetate extract, butanol extract, precipitate (D4), and the remaining aqueous phase. The part D4 containing soybean isoflavone glucosides was acquired and subjected to preparative HPLC for the isolation of target components. A preparative Nova-Pak HR C18 column (100 mm x 25 mm i. d. , 6 microm) was used in the preparation process. By isocratic elution with methanol-0.1% aqueous acetic acid (23:77, v/v) as the mobile phase at a flow rate of 20 mL/min, followed by concentration and desalination, three soybean isoflavone glucosides were obtained and subsequently identified by mass spectrometry as daidzin, glycitin, and genistin. HPLC analysis showed that the purities of the three soybean isoflavone glucosides were all higher than 99%.
建立了一种使用制备型反相高效液相色谱（RP-HPLC）从大豆总异黄酮提取物中分离大豆异黄酮糖苷的方法。通过溶剂萃取将总异黄酮提取物分为四部分，即乙酸乙酯提取物、丁醇提取物、沉淀（D4）和剩余水相。获得含有大豆异黄酮糖苷的D4部分，并对其进行制备型HPLC以分离目标成分。制备过程中使用了制备型Nova-Pak HR C18柱（100 mm×25 mm内径，6微米）。以甲醇-0.1%乙酸水溶液（23:77，v/v）作为流动相，以20 mL/min的流速进行等度洗脱，随后进行浓缩和脱盐，得到三种大豆异黄酮糖苷，随后通过质谱鉴定为黄豆苷、染料木苷和染料木素。HPLC分析表明，三种大豆异黄酮糖苷的纯度均高于99%。
