Lei Yujia, Gao Mingzhe, Wang Li, Xiao Hongbin
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Se Pu. 2010 Sep;28(9):902-4.
A new method for isolation of gentiopicroside (GPS) from Swertia mussotii Franch with reversed phase preparative high performance liquid chromatography (Pre-HPLC) is described. After 95% ethanol extract was obtained with solvent extraction and macroporous resin separation, a fraction containing GPS was acquired and subjected to Pre-HPLC for isolation of target component. The optimum operation parameters were selected as follows: a C18 column (200 mm x 50 mm, 5 microm), methanol-0.1% acetic water (30:70, v/v) as the mobile phase at flow rate of 75 mL/min, the detection wavelength at 254 nm, and the injection volume of 500 microL at the mass concentration of 225 g/L. The purity of the product was detected by HPLC method. The result showed that the purity of product was above 99%. The effective and rapid method has been successfully applied to the preparation of GPS from Swertia mussotii Franch.
描述了一种采用反相制备高效液相色谱法（Pre-HPLC）从川西獐牙菜中分离龙胆苦苷（GPS）的新方法。通过溶剂萃取和大孔树脂分离得到95%乙醇提取物后，获得了含GPS的馏分，并将其进行Pre-HPLC以分离目标成分。选择的最佳操作参数如下：C18柱（200 mm×50 mm，5μm），甲醇-0.1%乙酸水（30:70，v/v）作为流动相，流速为75 mL/min，检测波长为254 nm，进样体积为500μL，质量浓度为225 g/L。采用HPLC法检测产物纯度。结果表明产物纯度在99%以上。该有效且快速的方法已成功应用于从川西獐牙菜中制备GPS。
