Ou Yunfu, Yin Pinghe, Zhao Ling
Center of Experimental Technology, Jinan University, Guangzhou 510632, China.
Se Pu. 2006 Jul;24(4):411-3.
Sargassum hemiphyllum polysaccharides (SHP) was extracted from dry Sargassum hemiphyllum (Turner) C. Ag. powder using 60 - 80 degrees C purified water and then hydrolyzed with 4.0 g/L trifluoroacetic acid at 80 degrees C. Without any derivatization reaction, the determination of monosaccharides in SHP was developed by anion-exchange chromatography with pulsed amperometric detection with an Au working electrode and an Ag/AgCl reference electrode. Monosaccharides were separated on a CarboPac PA10 anion-column (2 mm i. d. x 250 mm) by using isocratic elution consisting of 14 mmol/L sodium hydroxide at a flow rate of 0.20 mL/min. Six monosaccharides, xylose, galactose, arabinose, glucose, rhamnose and fructose, contained in SHP were separated and determined. Their contents in SHP were 2 200, 820, 98, 4 560, 358 and 740 mg/kg, respectively. The recoveries of the six monosaccharides were in the range 86.0% - 108.0%. The detection limits for these monosaccharides ranged from 5.6 to 89.6 microg/kg. The experimental results showed that SHP mainly consisted of xylose and glucose with smaller quantities of galactose, arabinose, rhamnose and fructose. This method is suitable for the determination of monosaccharides without any derivatization reaction at the level of microg/kg in dry algae with high sensitivity and good precision.
半叶马尾藻多糖（SHP）是从干燥的半叶马尾藻（Turner）C. Ag. 粉末中用60 - 80℃的纯净水提取，然后在80℃下用4.0 g/L三氟乙酸水解。在不进行任何衍生化反应的情况下，采用阴离子交换色谱 - 脉冲安培检测法（以金工作电极和银/氯化银参比电极）对SHP中的单糖进行测定。单糖在CarboPac PA10阴离子柱（内径2 mm×250 mm）上分离，采用等度洗脱，洗脱液为14 mmol/L氢氧化钠，流速为0.20 mL/min。SHP中含有的木糖、半乳糖、阿拉伯糖、葡萄糖、鼠李糖和果糖六种单糖被分离并测定。它们在SHP中的含量分别为2200、820、98、4560、358和740 mg/kg。六种单糖的回收率在86.0% - 108.0%范围内。这些单糖的检测限在5.6至89.6 μg/kg之间。实验结果表明，SHP主要由木糖和葡萄糖组成，还含有少量的半乳糖、阿拉伯糖、鼠李糖和果糖。该方法适用于在不进行任何衍生化反应的情况下，对干藻中微克/千克水平的单糖进行测定，具有高灵敏度和良好的精密度。
