褐藻糖胶的化学特征分析及其免疫调节活性研究。

Chemical Characterization and Immunomodulatory Activity of Fucoidan from .

机构信息

College of Food Science and Technology, Guangdong Ocean University, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Marine Food, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Provincial Science and Technology Innovation Center for Subtropical Fruit and Vegetable Processing, Zhanjiang 524008, China.

Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, China.

出版信息

Mar Drugs. 2022 Dec 26;21(1):18. doi: 10.3390/md21010018.

Fucoidan is a sulfated algal polyanionic polysaccharide that possesses many biological activities. In this paper, a fucoidan (SHF) polysaccharide was extracted from collected in the South China Sea. The SHF, with a molecular weight of 1166.48 kDa (44.06%, /), consisted of glucose (32.68%, /), galactose (24.81%, /), fucose (20.75%, /), xylose (6.98%, /), mannose (2.76%, /), other neutral monosaccharides, and three uronic acids, including glucuronic acid (5.39%, /), mannuronic acid (1.76%, /), and guronuronic acid (1.76%, /). The SHF exhibited excellent immunostimulatory activity. An immunostimulating assay showed that SHF could significantly increase NO secretion in macrophage RAW 264.7 cells via upregulation of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) levels based on both gene expression and protein abundance. These results suggest that SHF isolated from has great potential to act as a health-boosting ingredient in the pharmaceutical and functional-food fields.

岩藻聚糖硫酸酯是一种具有多种生物活性的硫酸化海藻多糖。本文从南海采集的褐藻中提取了一种岩藻聚糖（SHF）多糖。SHF 的分子量为 1166.48 kDa（44.06%，/），由葡萄糖（32.68%，/）、半乳糖（24.81%，/）、岩藻糖（20.75%，/）、木糖（6.98%，/）、甘露糖（2.76%，/）、其他中性单糖和三种糖醛酸组成，包括葡萄糖醛酸（5.39%，/）、甘露糖醛酸（1.76%，/）和古罗糖醛酸（1.76%，/）。SHF 表现出优异的免疫刺激活性。免疫刺激试验表明，SHF 能够通过上调环氧化酶-2（COX-2）和诱导型一氧化氮合酶（iNOS）的水平，显著增加巨噬细胞 RAW 264.7 细胞中 NO 的分泌，这基于基因表达和蛋白丰度。这些结果表明，从中分离得到的 SHF 具有作为药物和功能性食品领域增强健康的成分的巨大潜力。