Crippen Tawni L, Sheffield Cynthia
Southern Plains Area Research Center, USDA-ARS, 2881 F and B Road, College Station, TX 77845, USA.
J Med Entomol. 2006 Sep;43(5):916-23. doi: 10.1603/0022-2585(2006)43[916:esdotl]2.0.co;2.
Understanding the dynamics of movement of bacteria within the environment and between species is crucial to unraveling the epidemiology of bacterial diseases and to developing biosecurity measures to prevent dissemination. Many arthropods, some beneficial and some detrimental, inhabit poultry houses. The lesser mealworm, Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae), is a pest commonly found in poultry litter that can harbor pathogens involved in both human and animal health issues. Current farm management practices perpetuate persistent infestations contributing to the dispersal of beetles and pathogens. To study the dissemination of bacteria by this beetle, we require the ability to differentiate internal from external sources of bacteria carried by the beetle. In this study, we tested previously described methods to externally disinfect beetles and found disinfectant efficacies between 40 and 98%. The irregular surface of the insect posed a challenge to cleansing procedures because the surface offered many recesses able to sequester bacteria. Complete bacterial disinfection was achieved with a serial treatment of ethanol and hydrogen peroxide or hydrogen peroxide/peracetic acid.
了解细菌在环境中以及物种之间的运动动态，对于揭示细菌性疾病的流行病学以及制定预防传播的生物安全措施至关重要。许多节肢动物栖息在禽舍中，有些有益，有些有害。黄粉虫（Alphitobius diaperinus (Panzer)，鞘翅目：拟步甲科）是一种常见于家禽粪便中的害虫，它可能携带涉及人类和动物健康问题的病原体。当前的农场管理做法使得持续性侵扰长期存在，这有助于甲虫和病原体的传播。为了研究这种甲虫传播细菌的情况，我们需要有能力区分甲虫携带的细菌的内部来源和外部来源。在本研究中，我们测试了先前描述的对外消毒甲虫的方法，发现消毒剂的有效率在40%至98%之间。昆虫表面不规则给清洁程序带来了挑战，因为表面有许多能够藏匿细菌的凹陷处。通过乙醇和过氧化氢或过氧化氢/过氧乙酸的系列处理实现了完全的细菌消毒。
