Groux-Muscatelli B, Bassaglia Y, Barritault D, Caruelle J P, Gautron J
Laboratoire de Biotechnologie des Cellules Eucaryotes, Université Paris-Val de Marne, Créteil, France.
Dev Biol. 1990 Dec;142(2):380-5. doi: 10.1016/0012-1606(90)90358-p.
Recent in vitro studies have indicated that the proliferation of satellite cells, which are involved in muscular regeneration in vivo, is stimulated by exogenous addition of fibroblast growth factor (FGF). We present evidence that satellite cell cultures produce acidic, but not basic FGF. Acidic or basic FGF content was measured by enzyme immunoassay on cellular extracts after partial purification by heparin-Sepharose chromatography. During maximal cell proliferation, the level of acidic fibroblast growth factor (aFGF) was increased over fivefold from the values obtained before plating. aFGF content drastically dropped at the postmitotic stage to almost the threshold of detection, and remained weak as differentiation was completed. The immunolocalization of aFGF using highly purified anti-aFGF antibodies confirmed these results and indicated that aFGF was cytoplasma- or membrane-associated. Our work suggests that an endogenous production of aFGF by satellite cells may trigger cell proliferation by an intra- or autocrine mechanism, and therefore play an important role in muscular regeneration.
最近的体外研究表明，参与体内肌肉再生的卫星细胞的增殖受到外源添加成纤维细胞生长因子（FGF）的刺激。我们提供的证据表明，卫星细胞培养物产生酸性FGF，但不产生碱性FGF。通过肝素-琼脂糖色谱部分纯化后，通过酶免疫测定法对细胞提取物中的酸性或碱性FGF含量进行测量。在细胞增殖达到最大值时，酸性成纤维细胞生长因子（aFGF）的水平比接种前的值增加了五倍多。有丝分裂后阶段，aFGF含量急剧下降至几乎检测阈值，并在分化完成后保持较低水平。使用高度纯化的抗aFGF抗体对aFGF进行免疫定位证实了这些结果，并表明aFGF与细胞质或细胞膜相关。我们的研究表明，卫星细胞内源性产生aFGF可能通过自分泌或旁分泌机制触发细胞增殖，因此在肌肉再生中起重要作用。
