Nakahara H, Watanabe K, Sugrue S P, Olsen B R, Caplan A I
Department of Biology, Case Western Reserve University, Cleveland, Ohio 44106.
Dev Biol. 1990 Dec;142(2):481-5. doi: 10.1016/0012-1606(90)90370-x.
Periosteal-derived cells of young chicks have been reported to possess the potential to undergo terminal differentiation into osteogenic or chondrogenic phenotypes under high cell density culture conditions. In this culture, the temporal and spatial distribution of type XII collagen was immunocytochemically assessed using a monoclonal antibody. These high-density plated cells first formed a multilayer of fibroblast-like cells, in which type I and XII collagen were evenly distributed throughout the full thickness of the culture. With time, the top portion of the culture differentiated into bone tissue, while cells below this top layer differentiated into hypertrophic chondrocytes. In this transition, type XII collagen was temporally and spatially colocalized primarily with type I collagen: the top portion of bone layer was positive for both type I and XII collagens, whereas their staining intensity in the bottom portion decreased with time in culture. Using this antibody, type XII collagen was also found in developing embryonic chick tibiotarsus. These observations, taken together, suggest that type XII collagen production is a characteristic property of bone-forming cells.
据报道，幼雏的骨膜来源细胞在高细胞密度培养条件下具有终末分化为成骨或软骨形成表型的潜力。在这种培养中，使用单克隆抗体通过免疫细胞化学方法评估了 XII 型胶原的时空分布。这些高密度接种的细胞首先形成一层成纤维细胞样细胞多层结构，其中 I 型和 XII 型胶原在培养物的整个厚度中均匀分布。随着时间的推移，培养物的顶部部分分化为骨组织，而该顶层下方的细胞分化为肥大软骨细胞。在这种转变过程中，XII 型胶原主要在时间和空间上与 I 型胶原共定位：骨层的顶部部分 I 型和 XII 型胶原均呈阳性，而它们在底部部分的染色强度随培养时间而降低。使用该抗体，在发育中的胚胎雏鸡胫跗骨中也发现了 XII 型胶原。综合这些观察结果，表明 XII 型胶原的产生是成骨细胞的一个特征性特性。
