Ghanem Ashraf
Biomedicinal Chemistry Unit, Biological and Medical Research Department, King Faisal Specialist Hospital and Research Centre MBC-03-95, P.O. Box 3354, Riyadh 11211, Saudi Arabia.
J Chromatogr A. 2006 Nov 3;1132(1-2):329-32. doi: 10.1016/j.chroma.2006.09.043. Epub 2006 Oct 2.
A set of racemic N-alkylated barbiturates were investigated for their direct enantioselective HPLC separation on a new chiral stationary phase (CSP) containing cellulose tris(3,5-dimethylphenylcarabamate) immobilized onto silica gel (Chiralpak IB) and its coated version (Chiralcel OD). They were online detected by UV and optical rotation detectors to trace the elution order of their enantiomers. Surprisingly, examples of false and true reversal of the elution order of enantiomers of barbiturates were observed and reported.
研究了一组外消旋N-烷基化巴比妥酸盐在一种新型手性固定相（CSP）上的直接对映体选择性高效液相色谱分离，该手性固定相包含固定在硅胶上的三（3,5-二甲基苯基氨基甲酸酯）纤维素（Chiralpak IB）及其涂覆版本（Chiralcel OD）。通过紫外和旋光检测器对它们进行在线检测，以追踪其对映体的洗脱顺序。令人惊讶的是，观察并报道了巴比妥酸盐对映体洗脱顺序的假反转和真反转实例。
