D'Hooge R, De Deyn P P, Verzwijvelen A, De Block J, De Potter W P
Laboratory of Neuropharmacology, University of Antwerp, Belgium.
Life Sci. 1990;47(20):1851-9. doi: 10.1016/0024-3205(90)90288-3.
The axonal transport and subcellular distribution of noradrenaline (NA), dopamine beta-hydroxylase (DBH) and neuropeptide Y (NPY) were determined in dog sciatic nerve using an accumulation technique. The results were compared with those obtained by application of the same procedures and methods on the splenic nerve in the same animal species. Evidence was found for the coexistence of NA and NPY in large dense-cored vesicles in dog sciatic nerve axons. After differential centrifugation and isopyenic sucrose density gradient centrifugation of 24 h ligated sciatic nerve pieces NA and NPY equilibrated around 1M sucrose. The DBH activity was dispersed broadly on the gradient. Subsequently, the accumulation of NA, DBH and NPY was studied in proximal and sital segments of 8, 12 and 24 h dog ligated sciatic nerve and inferences were made concerning the axonal transport of these compounds. NA, DBH and NPY displayed a divergent accumulation proximal to the ligation. After 12 h of ligation a transport rate was calculated of 4.8 +/- 1.8 mm/h for NA, of 5.9 +/- 1.5 mm/h for DBH and of 4.9 +/- 2.0 mm/h for NPY. With a correction for the stationary fractions, a similar fast transport rate of approximately 10 to 12 mm/h was proposed for NA, DBH and NPY. The occurrence was shown of a limited retrograde transport of DBH and possibly NPY, but not of NA.
采用蓄积技术测定了去甲肾上腺素（NA）、多巴胺β-羟化酶（DBH）和神经肽Y（NPY）在犬坐骨神经中的轴突运输及亚细胞分布情况。将结果与对同一动物物种的脾神经采用相同程序和方法所获得的结果进行了比较。发现犬坐骨神经轴突的大致密核心囊泡中存在NA和NPY共存的证据。对结扎24小时的坐骨神经节段进行差速离心和等密度蔗糖密度梯度离心后，NA和NPY在1M蔗糖左右达到平衡。DBH活性在梯度上分布较广。随后，研究了结扎8、12和24小时的犬坐骨神经近端和远端节段中NA、DBH和NPY的蓄积情况，并对这些化合物的轴突运输进行了推断。NA、DBH和NPY在结扎近端呈现出不同的蓄积情况。结扎12小时后，计算出NA的运输速率为4.8±1.8毫米/小时，DBH为5.9±1.5毫米/小时，NPY为4.9±2.0毫米/小时。校正固定部分后，提出NA、DBH和NPY具有相似的约10至12毫米/小时的快速运输速率。结果显示存在DBH以及可能存在NPY的有限逆行运输，但不存在NA的逆行运输。
