Schwaiger Stefan, Seger Christoph, Wiesbauer Bettina, Schneider Peter, Ellmerer Ernst P, Sturm Sonja, Stuppner Hermann
Institut für Pharmazie/Pharmakognosie, Leopold-Franzens-Universität Innsbruck, Innrain, Austria.
Phytochem Anal. 2006 Sep-Oct;17(5):291-8. doi: 10.1002/pca.917.
The analytical assessment of edelweiss (Leontopodium alpinum) herb extracts, used in traditional alpine medicine, has resulted in the development of a HPLC-PAD-MS method that allows baseline separation of almost all constituents. Peak assignment of 14 analytes was achieved by comparison of retention times, UV and mass spectra with those of reference compounds either commercially available (luteolin, apigenin and chlorogenic acid) or isolated from edelweiss plants by column chromatography. Ten of the isolated analytes were identified as the known natural products: quercetin-3-O-beta-D-glucoside, luteolin-7-O-beta-D-glucoside, luteolin-3'-O-beta-D-glucoside, luteolin-4'-O-beta-D-glucoside, apigenin-7-O-beta-D-glucoside, 6-hydroxy-luteolin-7-O-beta-D-glucoside, luteolin-7,4'-di-O-beta-D-glucoside, chrysoeriol-7-O-beta-D-glucoside, leontopodic acid and 3,5-dicaffeolyquinic acid. One analyte, 3,4,5-tri-(E)-caffeoly-D-glucaric acid proved to be a new natural product and was named leontopodic acid B. Structure elucidation was carried out by means of MS and NMR spectroscopy in all cases. The aerial plant parts of L. alpinum (capitula, inflorescence leaves, stems, stem leaves and leaves of the basal rosette) showed variable amounts of the above-mentioned constituents, although qualitative differences were not observable.
对传统高山医学中使用的雪绒花（高山火绒草）草本提取物进行的分析评估，促成了一种高效液相色谱 - 光电二极管阵列 - 质谱联用（HPLC - PAD - MS）方法的开发，该方法能够对几乎所有成分进行基线分离。通过将14种分析物的保留时间、紫外光谱和质谱与市售参考化合物（木犀草素、芹菜素和绿原酸）或通过柱色谱从雪绒花植物中分离得到的参考化合物进行比较，实现了峰的归属。所分离出的14种分析物中有10种被鉴定为已知的天然产物：槲皮素 - 3 - O - β - D - 葡萄糖苷、木犀草素 - 7 - O - β - D - 葡萄糖苷、木犀草素 - 3'- O - β - D - 葡萄糖苷、木犀草素 - 4'- O - β - D - 葡萄糖苷、芹菜素 - 7 - O - β - D - 葡萄糖苷、6 - 羟基 - 木犀草素 - 7 - O - β - D - 葡萄糖苷、木犀草素 - 7,4'-二 - O - β - D - 葡萄糖苷、 Chrysoeriol - 7 - O - β - D - 葡萄糖苷、火绒草酸和3,5 - 二咖啡酰奎宁酸。有一种分析物，3,4,5 - 三 - （E） - 咖啡酰 - D - 葡糖二酸被证明是一种新的天然产物，并被命名为火绒草酸B。在所有情况下，均通过质谱和核磁共振光谱进行结构解析。高山火绒草的地上部分（头状花序、花序叶、茎、茎生叶和基生莲座叶）显示出上述成分的含量各不相同，尽管未观察到定性差异。
