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利用超高效液相色谱/离子淌度-高分辨质谱联用技术分析金雀愈伤组织培养物中的多酚类化合物及其体外效应筛选

Profiling of Polyphenolic Compounds of Cass Callus Cultures Using UPLC/IM-HRMS and Screening of In Vitro Effects.

作者信息

Pralea Ioana-Ecaterina, Moldovan Radu-Cristian, Țigu Adrian-Bogdan, Petrache Alina-Maria, Hegheș Simona-Codruța, Mitoi Monica, Cogălniceanu Gina, Iuga Cristina-Adela

机构信息

Research Center for Advanced Medicine-MedFUTURE, Department of Proteomics and Metabolomics, "Iuliu Hațieganu" University of Medicine and Pharmacy Cluj-Napoca, Louis Pasteur Street 6, 400349 Cluj-Napoca, Romania.

Research Center for Advanced Medicine-MedFUTURE, Department of Translational Medicine, "Iuliu Hațieganu" University of Medicine and Pharmacy Cluj-Napoca, Louis Pasteur Street 6, 400349 Cluj-Napoca, Romania.

出版信息

Plants (Basel). 2021 Dec 29;11(1):100. doi: 10.3390/plants11010100.

DOI:10.3390/plants11010100
PMID:35009103
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8747157/
Abstract

Cass. (edelweiss) is recognized as a frequent constituent of anti-aging skin care products, providing increased antioxidant and anti-inflammatory defense. Considering the growing demand and the protected status of edelweiss in many countries, alternative methods of production have been developed, one of them being callus culturing. This study reports the phytochemical composition of a methanolic extract of callus cultures, characterized by liquid chromatography coupled to ion-mobility high resolution mass spectrometry (UPLC/IM-HRMS). The methanolic extract exhibited strong free radical scavenging activity (122.19 ± 7.28 mg AAE/g dw), while the quantitative evaluation revealed that four major constituents (phenylpropanoid derivatives) represent 57.13% (m/m) of the extract. Consequently, a screening of antiproliferative effects was performed on ten cancer cell lines, representative of prostate, colon, lung and breast cancer, showing inhibition of colony formation in all cases. These results provide a comprehensive phytochemical characterization of callus cultures using advanced IM-HRMS, while the in vitro explorations confirmed the potent antioxidant properties of edelweiss which are worth exploring further in cancer prevention.

摘要

高山火绒草被认为是抗衰老护肤品中常见的成分，具有增强抗氧化和抗炎防御的作用。鉴于对高山火绒草的需求不断增加以及其在许多国家的受保护地位，人们开发了替代生产方法，其中之一是愈伤组织培养。本研究报告了愈伤组织培养物甲醇提取物的植物化学成分，采用液相色谱-离子淌度高分辨率质谱联用（UPLC/IM-HRMS）进行表征。甲醇提取物表现出较强的自由基清除活性（122.19±7.28毫克抗坏血酸当量/克干重），而定量评估显示四种主要成分（苯丙烷类衍生物）占提取物的57.13%（质量/质量）。因此，对代表前列腺癌、结肠癌、肺癌和乳腺癌的十种癌细胞系进行了抗增殖作用筛选，结果显示在所有情况下均能抑制集落形成。这些结果利用先进的IM-HRMS对愈伤组织培养物进行了全面的植物化学表征，而体外研究证实了高山火绒草具有强大的抗氧化特性，值得在癌症预防方面进一步探索。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd03/8747157/b217d4933a02/plants-11-00100-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd03/8747157/c4c3c28771b6/plants-11-00100-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd03/8747157/811a4d8c5aec/plants-11-00100-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd03/8747157/b217d4933a02/plants-11-00100-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd03/8747157/c4c3c28771b6/plants-11-00100-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd03/8747157/811a4d8c5aec/plants-11-00100-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd03/8747157/b217d4933a02/plants-11-00100-g003.jpg

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