Suskov I N, Agadzhanian A V, Kuz'mina N S, Elisova T V, Iofa E L, Nilova I N, Akaeva E A, Kuznetsova G I, Rubanovich A V, Tskhovrebova L V, Baleva L S, Sipiagina A E
Radiats Biol Radioecol. 2006 Jul-Aug;46(4):466-74.
A complex genetic examination of children which belong to two cohorts and their parents were carried out. The first cohort included children and constantly living on territories contaminated with radionuclides (Novozybkov district, Bryansk region). They were subdivided in groups according to the ontogenetic age periods of development of their parents at the time of the Chernobyl accident. In the children born in 1986-1995 the level of aberrant genomes is significantly higher as compared to the control (p < 0.001). In children born in 1998-2002 the differences are insignificant (p > 0.05). The frequency of aberrant genomes had a tendency to decrease with the period of time between the birth date of a child and the moment of the accident. Analysis of the results of cytogenetic investigation for the same living on territories with different densities of radioactive contamination (zone I-- 627-688 kBq/m2, 137Cs and zone II-- 135-402 kBq/m2, 137Cs) revealed insignificant differences in the spectrum and average frequencies of chromosome aberrations. The second cohort included children born in 1987-1991 and 1993-2002 from irradiated fathers (Chernobyl clean-up workers) and unirradiated mothers living on territories without radionuclide contamination. These children also displayed increased frequencies of aberrant genomes as compared to the control (p < 0.001). The analysis of the dynamics years of birth of cytogenetic disturbances in the same cohorts of children showed the average frequencies of aberrant genomes remain higher than the control level. In most of the children of both cohorts the repair synthesis of genome DNA by gamma- and UV-radiation is reduced as compared to one in the children from the control group.
对来自两个队列的儿童及其父母进行了复杂的基因检测。第一个队列包括长期生活在受放射性核素污染地区（布良斯克州新兹布科夫区）的儿童。根据切尔诺贝利事故发生时其父母的个体发育年龄阶段，将他们分成不同组。与对照组相比，1986 - 1995年出生的儿童中异常基因组水平显著更高（p < 0.001）。1998 - 2002年出生的儿童中差异不显著（p > 0.05）。异常基因组的频率有随着儿童出生日期与事故发生时刻之间的时间间隔而降低的趋势。对生活在不同放射性污染密度地区（I区——627 - 688 kBq/m²，¹³⁷Cs；II区——135 - 402 kBq/m²，¹³⁷Cs）的同一批儿童的细胞遗传学研究结果分析显示，染色体畸变的谱型和平均频率差异不显著。第二个队列包括1987 - 1991年和1993 - 2002年出生的儿童，他们的父亲是受辐射者（切尔诺贝利清理工人），母亲未受辐射，生活在无放射性核素污染的地区。与对照组相比，这些儿童也表现出异常基因组频率增加（p < 0.001）。对同一批儿童细胞遗传学紊乱的出生年份动态分析表明，异常基因组的平均频率仍高于对照水平。与对照组儿童相比，两个队列中的大多数儿童经γ射线和紫外线辐射后基因组DNA的修复合成减少。
