Kumari H L, Shuler C, Mannix D G, Milo G E
Department of Physiological Chemistry, Ohio State University College of Medicine, Columbus 43210.
Exp Mol Pathol. 1990 Oct;53(2):167-79. doi: 10.1016/0014-4800(90)90041-b.
High molecular weight DNA, which was isolated from a chondrosarcoma cell line, was transfected into human neonatal foreskin fibroblasts and NIH/3T3 cells. Both types of transfected cells expressed anchorage-independent growth in soft agar and produced tumors in nude mice. The tumors which developed in nude mice following injection of transfected human fibroblasts grew to approximately 0.8 cm in diameter in four weeks. The tumors which developed from transfected NIH/3T3 cells grew to greater than 2.0 cm in diameter in 6 weeks. After growth in soft agar the transfected human fibroblasts expressed a cell surface sarcoma-associated epitope recognized by the monoclonal antibody 345.134S. In addition to the transfected human fibroblasts, the original human chondrosarcoma tumor, the chondrosarcoma cell line derived from the tumor, and the nude mouse tumor which developed from transfected human fibroblasts all exhibited positive reactivity with the monoclonal antibody 345.134S. Transfected NIH/3T3 cells that exhibited anchorage-independent growth and tumorigenicity did not exhibit detectable reactivity with the monoclonal antibody. These results suggest that the expression of the tumor-associated cell surface antigen appears to be an early event correlated with transformation of the transfected human cells but not directly related to the tumorigenic potential of the DNA. The transfected cells which expressed anchorage independent growth exhibited the sarcoma cell surface antigen prior to attaining the potential for tumorigenicity.
从软骨肉瘤细胞系中分离出的高分子量DNA被转染到人新生儿包皮成纤维细胞和NIH/3T3细胞中。两种类型的转染细胞在软琼脂中均表现出不依赖贴壁的生长，并在裸鼠体内产生肿瘤。注射转染的人成纤维细胞后在裸鼠体内形成的肿瘤在4周内直径增长到约0.8厘米。由转染的NIH/3T3细胞形成的肿瘤在6周内直径增长到超过2.0厘米。在软琼脂中生长后，转染的人成纤维细胞表达了一种细胞表面肉瘤相关表位，该表位可被单克隆抗体345.134S识别。除了转染的人成纤维细胞外，原始的人软骨肉瘤肿瘤、从该肿瘤衍生的软骨肉瘤细胞系以及由转染的人成纤维细胞形成的裸鼠肿瘤均与单克隆抗体345.134S表现出阳性反应。表现出不依赖贴壁生长和致瘤性的转染NIH/3T3细胞与单克隆抗体未表现出可检测到的反应性。这些结果表明，肿瘤相关细胞表面抗原的表达似乎是与转染的人细胞转化相关的早期事件，但与DNA的致瘤潜力无直接关系。表现出不依赖贴壁生长的转染细胞在获得致瘤潜力之前就表现出肉瘤细胞表面抗原。
