Kumari H L, Shuler C F, Lehman T, Ferrone S, Milo G E
Ohio State University Comprehensive Cancer Center, Columbus 43210.
Carcinogenesis. 1989 Feb;10(2):401-4. doi: 10.1093/carcin/10.2.401.
DNA isolated from chondrosarcoma cells effectively transformed NIH-3T3 cells and human foreskin fibroblasts. The transfected NIH-3T3 cells, directly implanted three or four passages later, formed progressively growing tumors (greater than or equal to 2.0 cm in diameter) subcutaneously in nude mice. No metastasis was evident upon pathological examination of the tumor bearing mice. Transfected human foreskin fibroblasts that exhibited anchorage independent growth formed only small tumors in nude mice (less than 0.6 cm in diameter). The transfected human cells which exhibited anchorage independent growth reacted with the monoclonal antibody 345.134S, specific for an epitope expressed by human sarcoma cells. The transfected NIH-3T3 cells did not exhibit reactivity with the same monoclonal antibody. Southern blot analysis of the DNA prepared from the transfected NIH-3T3 cells, that developed as a progressively growing tumor in a nude mouse, revealed the presence of human repetitive DNA sequences.
从软骨肉瘤细胞中分离出的DNA有效地转化了NIH-3T3细胞和人包皮成纤维细胞。转染后的NIH-3T3细胞在三到四代后直接植入，在裸鼠皮下形成了逐渐生长的肿瘤（直径大于或等于2.0厘米）。对荷瘤小鼠进行病理检查时未发现转移迹象。表现出不依赖贴壁生长的转染人包皮成纤维细胞在裸鼠中仅形成小肿瘤（直径小于0.6厘米）。表现出不依赖贴壁生长的转染人细胞与单克隆抗体345.134S发生反应，该抗体对人肉瘤细胞表达的表位具有特异性。转染后的NIH-3T3细胞与同一单克隆抗体不发生反应。对在裸鼠中形成逐渐生长肿瘤的转染NIH-3T3细胞制备的DNA进行Southern印迹分析，结果显示存在人重复DNA序列。
