Kucharska Anna, Górska Elzbieta, Popko Katarzyna, Wasik Maria, Rymkiewicz-Kluczyńska Barbara
Katedra i Klinika Pediatrii and Endokrynologii AM w Warszawie, Warszawa.
Endokrynol Diabetol Chor Przemiany Materii Wieku Rozw. 2006;12(3):167-70.
The cytotoxic T lymphocyte-associated antigen-4 (CTLA-4) is a transmembrane molecule present on T cells that plays a critical role in the down regulation of antigen-activated immune response. Its gene polymorphism is a strong candidate gene for conferring susceptibility to thyroid autoimmunity. THE AIM of the study was to analyse the surface CTLA-4 (CD 152) expression on peripheral blood lymphocytes.
Blood samples from 66 children were analysed: 36 with Hashimoto's thyroiditis and 30 healthy, age-matched controls, mean age: 13.4 years. The percentages of peripheral blood lymphocyte subsets were analysed by evaluation of CD antigens surface expression with three colour flow cytometry using Coulter EPICS XL cytometer. CD28, CD152, CD4, CD8 antigens were evaluated. Statistical analysis was performed using t- test and other tests of the Statistica programme. P-value <0.05 was considered as statistically significant.
In children with Hashimoto's thyroiditis the surface expression of CD8(+) CD152(+) T cells was statistically significant lower than in healthy controls. P-value was 0,02. Percentage of other CD examined in the study did not differ significantly in patients and in the control group.
In children with Hashimoto's thyroiditis the surface expression of CTLA-4 (CD152) on peripheral blood lymphocytes CD8(+) was statistically significantly lower than in healthy controls.
细胞毒性T淋巴细胞相关抗原4（CTLA-4）是一种存在于T细胞上的跨膜分子，在抗原激活的免疫反应下调中起关键作用。其基因多态性是导致甲状腺自身免疫易感性的一个强有力的候选基因。本研究的目的是分析外周血淋巴细胞表面CTLA-4（CD152）的表达情况。
分析了66名儿童的血样：36名患有桥本甲状腺炎，30名健康且年龄匹配的对照儿童，平均年龄为13.4岁。使用库尔特EPICS XL细胞仪通过三色流式细胞术评估CD抗原表面表达，分析外周血淋巴细胞亚群的百分比。评估CD28、CD152、CD4、CD8抗原。使用t检验和Statistica程序的其他检验进行统计分析。P值<0.05被认为具有统计学意义。
在患有桥本甲状腺炎的儿童中，CD8(+) CD152(+) T细胞的表面表达在统计学上显著低于健康对照组。P值为0.02。本研究中检测的其他CD的百分比在患者和对照组中没有显著差异。
在患有桥本甲状腺炎的儿童中，外周血淋巴细胞CD8(+)上CTLA-4（CD152）的表面表达在统计学上显著低于健康对照组。
