Florys Bozena, Głowińska Barbara, Urban Mirosława, Peczyńska Jadwiga
II Klinika Chorób Dzieci w Białymstoku, Białystok.
Endokrynol Diabetol Chor Przemiany Materii Wieku Rozw. 2006;12(3):184-9.
Abnormal activation of the matrix metalloproteinases (MMPs) in diabetes mellitus leads to extracellular matrix changes through the structural protein composition changes. The metalloproteinases inhibitors (TIMPs) are regulatory factors in this activity. Not all regulating mechanisms are completely known, especially in patients with type 1 diabetes.
evaluation of MMP-2, MMP-9 and TIMP-1, TIMP-2 levels in children and adolescents with type 1 diabetes.
74 patients in the mean age 15 years (+/-3.0) suffering from type 1 diabetes for mean 6.6 years (+/-3.6) took part in the study. Patients were treated with flexible multiple daily insulin (n=54) and with continuous subcutaneous insulin infusion - personal insulin pump (n=20). MMP-2, MMP-9, TIMP-1 and TIMP-2 blood serum levels were measured in all patients. 45 healthy persons matched for age, without atherosclerosis risk factors, with proper BMI and lipids levels were in the control group.
MMP-2 level as well as TIMP-2 and TIMP-2 levels were significantly higher in patients with type 1 diabetes in comparison to the control group (p respectively <0,01; <0,02; <0,001). We observed higher MMP-9 level in obese patients than in patients with BMI value below 90 pc for sex and age (p<0,02). We noted lower MMP-2 level in patients with chronic complications and/or arterial hypertension (n=24) in comparison to patients without that kind of complications (p<0,05). Positive correlation between TIMP-1 level and HbA1c level was noted. Age of patients as well as BMI negatively correlated with MMP-2 and TIMP-2 and positively with TIMP-1. We observed a correlation between MMP-2, TIMP-2 and especially TIMP-1 with lipid levels. Strong positive correlation was noted between MMP-2 and TIMP-2 (r=0.8; p<0,0001).
糖尿病患者体内基质金属蛋白酶（MMPs）的异常激活会通过结构蛋白组成的改变导致细胞外基质变化。金属蛋白酶抑制剂（TIMPs）是这一活动的调节因子。并非所有调节机制都完全清楚，尤其是在1型糖尿病患者中。
评估1型糖尿病儿童和青少年的MMP - 2、MMP - 9以及TIMP - 1、TIMP - 2水平。
74名平均年龄15岁（±3.0）、患1型糖尿病平均6.6年（±3.6）的患者参与了研究。患者分别接受灵活的每日多次胰岛素治疗（n = 54）和持续皮下胰岛素输注——个人胰岛素泵治疗（n = 20）。测量了所有患者血清中的MMP - 2、MMP - 9、TIMP - 1和TIMP - 2水平。对照组为45名年龄匹配、无动脉粥样硬化危险因素、BMI和血脂水平正常的健康人。
与对照组相比，1型糖尿病患者的MMP - 2水平以及TIMP - 2和TIMP - 2水平显著更高（p值分别<0.01；<0.02；<0.001）。我们观察到肥胖患者的MMP - 9水平高于BMI值低于同性别和年龄组第90百分位数的患者（p<0.02）。与无此类并发症的患者相比，患有慢性并发症和/或动脉高血压的患者（n = 24）的MMP - 2水平较低（p<0.05）。观察到TIMP - 1水平与糖化血红蛋白（HbA1c）水平呈正相关。患者年龄以及BMI与MMP - 2和TIMP - 2呈负相关，与TIMP - 1呈正相关。我们观察到MMP - 2、TIMP - 2尤其是TIMP - 1与血脂水平之间存在相关性。MMP - 2与TIMP - 2之间存在强正相关（r = 0.8；p<0.0001）。
