Głowińska-Olszewska Barbara, Urban Mirosława
2nd Department of Children's Diseases, Medical University of Białystok, 15-274 Białystok, Poland.
Metabolism. 2007 Jun;56(6):799-805. doi: 10.1016/j.metabol.2007.01.011.
Matrix metalloproteinases (MMPs) have been implicated in the atherosclerotic process and risk factors for the disease such as hypertension, hyperlipidemia, or diabetes mellitus in adults. So far, circulating levels of MMPs and their tissue inhibitors (TIMPs) have not been assessed in children and adolescents with obesity, a known risk factor for cardiovascular disease. Plasma levels of MMP-9 and TIMP-1 were measured immunoenzymatically in 45 obese children and adolescents, aged 15 +/- 1.8 years. The control group consisted of 28 healthy children, aged 14.5 +/- 2.5 years. MMP-9 and TIMP-1 concentrations were higher in obese children than in the control group (MMP-9: 553.5 +/- 311 vs 400.4 +/- 204 ng/mL, respectively; P = .02; TIMP-1: 161.2 +/- 32 vs 143.1 +/- 20.1 ng/mL, respectively; P = .03). We found significantly higher levels of MMP-9 in obese children with coexisting hypertension than in obese normotensive patients (635 +/- 308 vs 450 +/- 289 ng/mL, respectively; P = .04). MMP-9 correlated with body mass index (BMI) (r = 0.33, P = .005) and fasting insulin (r = 0.3, P = .013); TIMP-1 correlated with BMI (r = 0.35, P = .006). In the group of obese hypertensive children (n = 25), MMP-9 correlated with BMI (r = 0.41, P = .001), systolic blood pressure (r = 0.41, P = .002), fasting insulin (r = 0.37, P = .006), and homeostasis model assessment index of insulin resistance (r = 0.27, P = .03). TIMP-1 correlated with BMI (r = 0.33, P = .025) and systolic (r = 0.38, P = .008) and diastolic (r = 0.47, P = .001) blood pressure. In the regression models, MMP-9 was found to be dependent on fasting insulin (R(2) = 0.16, P = .04), and TIMP-1 on BMI (R(2) = 0.14, P = .04). In the obese hypertensive group, TIMP-1 was dependent on diastolic blood pressure (R(2) = 0.18, P = .04). Obese children and adolescents have elevated plasma concentrations of MMP-9 and TIMP-1. Coexistence of hypertension may exacerbate alterations of extracellular matrix turnover in these patients. It might be hypothesized that elevated MMP and TIMP concentrations may be related to increased cardiovascular risk in obese and particularly in obese hypertensive children and adolescents.
基质金属蛋白酶（MMPs）与动脉粥样硬化进程以及该疾病的危险因素有关，如成年人的高血压、高脂血症或糖尿病。到目前为止，尚未对肥胖（已知的心血管疾病危险因素）儿童和青少年的MMPs及其组织抑制剂（TIMPs）的循环水平进行评估。采用免疫酶法测量了45名15±1.8岁肥胖儿童和青少年的血浆MMP-9和TIMP-1水平。对照组由28名14.5±2.5岁的健康儿童组成。肥胖儿童的MMP-9和TIMP-1浓度高于对照组（MMP-9：分别为553.5±311与400.4±204 ng/mL；P = 0.02；TIMP-1：分别为161.2±32与143.1±20.1 ng/mL；P = 0.03）。我们发现，并存高血压的肥胖儿童的MMP-9水平显著高于血压正常的肥胖患者（分别为6
