Moubasher A H, Abdel-Hafez S I, Shoreit A A, Ismail M A
Botany Department, Faculty of Science, Assiut University, Egypt.
Folia Microbiol (Praha). 1990;35(4):298-310. doi: 10.1007/BF02821282.
Three methods were used for the isolation of fungi in the present investigation: the dilution-plate method, hair-baiting technique with horse hairs and the "exposed plate" method. Sabouraud's glucose agar at 28 degrees C was also used as isolation medium. 102 species and 2 species varieties belonging to 36 genera were collected from combine harvester wheat and sorghum dusts and from the atmosphere of hay or winnow sites. Chrysosporium was represented by 6 species: C. asperatum, C. indicum, C. keratinophilum, C. merdarium, C. pannorum and C. tropicum. Several filamentous fungi tolerating high levels of cycloheximide and other moulds were frequently encountered in the two types of dusts and in the two atmospheres, such as members of Acremonium, Alternaria, Aspergillus, Cladosporium, Emericella, Eupenicillium, Fusarium, Paecilomyces, Penicillium, Piedraia, Rhizopus, Scopulariopsis and others.
稀释平板法、马毛诱菌技术和“暴露平板”法。28℃的沙氏葡萄糖琼脂也用作分离培养基。从联合收割机收获的小麦和高粱粉尘以及干草或扬谷场地的空气中收集到属于36个属的102个种和2个变种。金孢子菌属有6个种:粗糙金孢子菌、印度金孢子菌、嗜角质金孢子菌、粪生金孢子菌、泛生金孢子菌和热带金孢子菌。在这两类粉尘和两种环境空气中经常遇到几种耐受高浓度放线菌酮的丝状真菌和其他霉菌,如枝顶孢属、链格孢属、曲霉属、枝孢属、谢瓦散囊菌属、优美青霉属(拟青霉属)、镰刀菌属、拟青霉属、青霉属、毛孢子菌属、根霉属、帚霉属等的成员。