Rodriguez F, Tocanne J F, Lopez A
Institut de Biologie Cellulaire et de Génétique du CNRS, 118 Route de Narbonne, 31062 Toulouse Cedex, France.
Biophys Chem. 1995 Feb;53(3):169-80. doi: 10.1016/0301-4622(94)00109-w.
When studying lipid-lipid or lipid-protein interaction in membranes, the correct interpretation of data obtained when using fluorescent phospholipid probes requires the best possible knowledge of probe behaviour in phospholipid membranes. Analysis of the translational dynamics and photochemical properties of the anthracene-labeled phosphatidylcholine (EAPC) shows that a self-association process occurs with this probe in the membrane at the ground state. This anthracene self-association is characterized and leads to a hypochromic effect which has been studied by means of ultraviolet absorption spectroscopy in unilamellar egg-yolk phosphatidylcholine (EggPC) vesicles. A model with indefinite linear self-association, in which each step has the same equilibrium constant, best describes the data. The equilibrium constant was found to be in the 300-500 M(-1) range and the complex lateral distribution pattern of EAPC in model membranes, which results from this self-association process, is characterized and seems to be mainly controlled by the amount of EAPC incorporated into the lipid bilayer.
在研究膜中的脂质-脂质或脂质-蛋白质相互作用时，要正确解读使用荧光磷脂探针获得的数据，就需要尽可能深入了解探针在磷脂膜中的行为。对蒽标记的磷脂酰胆碱（EAPC）的平移动力学和光化学性质的分析表明，该探针在基态下于膜中会发生自缔合过程。这种蒽自缔合的特性得以表征，并导致了减色效应，已通过紫外吸收光谱法在单层蛋黄磷脂酰胆碱（EggPC）囊泡中对其进行了研究。一个具有无限线性自缔合的模型（其中每个步骤具有相同的平衡常数）能最好地描述这些数据。发现平衡常数在300 - 500 M⁻¹范围内，并且由这种自缔合过程导致的EAPC在模型膜中的复杂横向分布模式得以表征，且似乎主要受掺入脂质双层中的EAPC量的控制。
