Harding S E
University of Nottingham, School of Agriculture, Sutton Bonington LE12 5RD, UK.
Biophys Chem. 1995 Jun-Jul;55(1-2):69-93. doi: 10.1016/0301-4622(94)00143-8.
Hydrodynamics provides a powerful complementary role to the traditional "high resolution" techniques for the investigation of macromolecular conformation, especially in dilute solution, conditions which are generally inaccessible to other structural techniques. This paper describes the state of art of hydrodynamic representations for macromolecular conformation, in terms of (1) simple but straightforward ellipsoid of revolution modelling; (2) general triaxial ellipsoid modelling; (3) hydrodynamic bead modelling; (4) the ability, especially for polydisperse macromolecular systems, to distinguish between various conformation types; (5) analysis of macromolecular flexibility.
流体动力学为研究大分子构象的传统“高分辨率”技术提供了强有力的补充作用，特别是在稀溶液中，而其他结构技术通常难以达到这种条件。本文从以下几个方面描述了大分子构象的流体动力学表示的现状：（1）简单直接的旋转椭球体建模；（2）一般的三轴椭球体建模；（3）流体动力学珠子建模；（4）特别是对于多分散大分子系统，区分各种构象类型的能力；（5）大分子柔韧性分析。
