生物技术中多分散大分子系统流体动力学表征的现代方法综述。
A review of modern approaches to the hydrodynamic characterisation of polydisperse macromolecular systems in biotechnology.
作者信息
Gillis Richard B, Rowe Arthur J, Adams Gary G, Harding Stephen E
机构信息
a National Centre for Macromolecular Hydrodynamics, Sutton Bonington Campus, University of Nottingham , Sutton Bonington , LE12 5RD , UK.
出版信息
Biotechnol Genet Eng Rev. 2014 Oct;30(1-2):142-57. doi: 10.1080/02648725.2014.994870. Epub 2015 Feb 16.
This short review considers the range of modern techniques for the hydrodynamic characterisation of macromolecules - particularly large glycosylated systems used in the food, biopharma and healthcare industries. The range or polydispersity of molecular weights and conformations presents special challenges compared to proteins. The review is aimed, without going into any great theoretical or methodological depth, to help the Industrial Biotechnologist choose the appropriate methodology or combination of methodologies for providing the detail he/she needs for particular applications.
本简短综述探讨了用于大分子流体动力学表征的一系列现代技术，特别是食品、生物制药和医疗保健行业中使用的大型糖基化系统。与蛋白质相比，分子量和构象的范围或多分散性带来了特殊挑战。本综述旨在帮助工业生物技术专家选择合适的方法或方法组合，而无需深入任何重大的理论或方法深度，以提供其特定应用所需的详细信息。
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