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灰色链霉菌中抗肿瘤脱氧糖途径的组合生物合成：用于四种2,6 -二脱氧己糖生物合成的“非天然天然基因簇”的重构

Combinatorial biosynthesis of antitumor deoxysugar pathways in Streptomyces griseus: Reconstitution of "unnatural natural gene clusters" for the biosynthesis of four 2,6-D-dideoxyhexoses.

作者信息

Pérez María, Lombó Felipe, Baig Irfan, Braña Alfredo F, Rohr Jürgen, Salas José A, Méndez Carmen

机构信息

Departamento de Biología Funcional e Instituto Universitario de Oncología del Principado de Asturias (I.U.O.P.A), Universidad de Oviedo, Oviedo, Spain.

出版信息

Appl Environ Microbiol. 2006 Oct;72(10):6644-52. doi: 10.1128/AEM.01266-06.

DOI:10.1128/AEM.01266-06
PMID:17021216
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1610316/
Abstract

Combinatorial biosynthesis was applied to Streptomyces deoxysugar biosynthesis genes in order to reconstitute "unnatural natural gene clusters" for the biosynthesis of four D-deoxysugars (D-olivose, D-oliose, D-digitoxose, and D-boivinose). Expression of these gene clusters in Streptomyces albus 16F4 was used to prove the functionality of the designed clusters through the generation of glycosylated tetracenomycins. Three glycosylated tetracenomycins were generated and characterized, two of which (D-digitoxosyl-tetracenomycin C and D-boivinosyl-tetracenocmycin C) were novel compounds. The constructed gene clusters may be used to increase the capabilities of microorganisms to synthesize new deoxysugars and therefore to produce new glycosylated bioactive compounds.

摘要

组合生物合成被应用于链霉菌脱氧糖生物合成基因，以重建用于四种D-脱氧糖（D-橄榄糖、D-油糖、D-洋地黄毒糖和D-博伊文糖）生物合成的“非天然天然基因簇”。这些基因簇在白色链霉菌16F4中的表达用于通过糖基化四环素的产生来证明设计的基因簇的功能。产生并表征了三种糖基化四环素，其中两种（D-洋地黄毒糖基-四环素C和D-博伊文糖基-四环素C）是新化合物。构建的基因簇可用于提高微生物合成新脱氧糖的能力，从而生产新的糖基化生物活性化合物。

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