Stockmann M, Piepersberg W
Bergische Universität GH, Wuppertal, F.R.G.
FEMS Microbiol Lett. 1992 Jan 1;69(2):185-9. doi: 10.1016/0378-1097(92)90626-y.
DNA probes were designed from the streptomycin production genes strDELM of Streptomyces griseus involved in the biosynthesis of the 6-deoxyhexose (6DOH) dihydrostreptose which could detect the genomic fragments coding for 6DOH formation in other actinomycetes strains. In about 70% of the 43 strains tested at least one signal could be detected with strD-, strE- or strLM-specific probes. Evidence is presented that the hybridizing genes are mostly clustered and probably engaged in the formation of secondary metabolites. Because of the wide-spread use of 6DOH constituents in natural products these probes should allow to detect a vast array of different secondary metabolic gene clusters in actinomycetes.
从参与6-脱氧己糖（6DOH）二氢链霉素生物合成的灰色链霉菌的链霉素生产基因strDELM设计了DNA探针，该探针可检测其他放线菌菌株中编码6DOH形成的基因组片段。在所测试的43个菌株中，约70%至少能用strD、strE或strLM特异性探针检测到一个信号。有证据表明，杂交基因大多成簇存在，可能参与次级代谢产物的形成。由于6DOH成分在天然产物中的广泛应用，这些探针应能检测放线菌中大量不同的次级代谢基因簇。
