Urakawa Hidetoshi, Maki Hideaki, Kawabata Sumiko, Fujiwara Taketomo, Ando Haruo, Kawai Toshio, Hiwatari Takehiko, Kohata Kunio, Watanabe Masataka
National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki 305-8506, Japan.
Appl Environ Microbiol. 2006 Oct;72(10):6845-50. doi: 10.1128/AEM.00807-06.
A polyphasic, culture-independent study was conducted to investigate the abundance and population structure of ammonia-oxidizing bacteria (AOB) in canal sediments receiving wastewater discharge. The abundance of AOB ranged from 0.2 to 1.9% and 1.6 to 5.7% of the total bacterial fraction by real-time PCR and immunofluorescence staining, respectively. Clone analysis and restriction endonuclease analysis revealed that the AOB communities influenced by the wastewater discharge were dominated by Nitrosomonas, were similar to each other, and were less diverse than the communities outside of the immediate discharge zone.
开展了一项多相、不依赖培养的研究,以调查接收废水排放的运河沉积物中氨氧化细菌(AOB)的丰度和种群结构。通过实时PCR和免疫荧光染色法,AOB的丰度分别占细菌总数的0.2%至1.9%和1.6%至5.7%。克隆分析和限制性内切酶分析表明,受废水排放影响的AOB群落以亚硝化单胞菌属为主,群落之间相似,且与紧邻排放区外的群落相比多样性较低。