Institute of Marine Resources, A-018, Scripps Institution of Oceanography, University of California, San Diego, La Jolla, California 92093.
Appl Environ Microbiol. 1985 Aug;50(2):194-201. doi: 10.1128/aem.50.2.194-201.1985.
Immunofluorescence assays for marine ammonium- and nitrite-oxidizing bacteria were used to assess the diversity of nitrifying bacteria isolated from marine environments. The antisera show relatively broad specificity, in that each reacts with several strains of the same physiological type as the strain to which the antiserum was prepared. The antisera do not, however, react with any strains of differing physiological type. Seventy percent of the 30 unidentified ammonium-oxidizing isolates tested reacted with one or both of the antisera produced to marine ammonium-oxidizing strains, and 8 of the 9 unidentified nitrite-oxidizing strains tested reacted with 1 or more of the 3 nitrite oxidizer antisera used. Ammonium- and nitrite-oxidizing bacteria were enumerated in samples taken in a depth profile (to 750 m) in the Southern California Bight by immunofluorescence assays for two ammonium oxidizers and two nitrite oxidizers. Average abundances of the two types of nitrifiers were 3.5 x 10 and 2.8 x 10 cells liter, respectively. Nitrifiers constitute 0.1 to 0.8% of the total bacterial population in these samples.
免疫荧光测定法被用于检测从海洋环境中分离出的海洋氨氧化菌和亚硝酸盐氧化菌的多样性。抗血清显示出相对广泛的特异性，因为每种抗血清都与同一生理类型的几种菌株发生反应，就像与制备抗血清的菌株一样。然而，抗血清不会与任何不同生理类型的菌株发生反应。在测试的 30 种未鉴定的氨氧化菌中，有 70%与针对海洋氨氧化菌产生的一种或两种抗血清发生反应，在测试的 9 种未鉴定的亚硝酸盐氧化菌中，有 8 种与使用的 3 种亚硝酸盐氧化菌抗血清中的 1 种或多种发生反应。通过针对两种氨氧化菌和两种亚硝酸盐氧化菌的免疫荧光测定法，对南加州湾区的深度剖面（至 750 米）中的样本进行了氨氧化菌和亚硝酸盐氧化菌的计数。这两种硝化菌的平均丰度分别为 3.5 x 10 和 2.8 x 10 细胞/升。硝化菌占这些样本中总细菌数量的 0.1%至 0.8%。
