Department of Ecology and Environmental Studies, Florida Gulf Coast University, Fort Myers, FL, USA.
Department of Civil and Environmental Engineering, University of Washington, Seattle, WA, USA.
Arch Microbiol. 2022 Aug 17;204(9):560. doi: 10.1007/s00203-022-03173-5.
A betaproteobacterial chemolithotrophic ammonia-oxidizing bacterium designated APG5 was isolated from supralittoral sand of the Edmonds City Beach, WA, USA. Growth was observed at 10-35 °C (optimum, 30 °C), pH 5-9 (optimum, pH 8) and ammonia concentrations as high as 100 mM (optimum, 1-30 mM NHCl). The strain grows optimally in a freshwater medium but tolerates up to 400 mM NaCl. It is most closely related to 'Nitrosomonas ureae' (96.7% 16S rRNA and 92.4% amoA sequence identity). The 3.75-Mbp of AGP5 draft genome contained a single rRNA operon and all necessary tRNA genes and has the lowest G+C content (43.5%) when compared to the previously reported genomes of reference strains in cluster 6 Nitrosomonas. Based on an average nucleotide identity of 82% with its closest relative ('N. ureae' Nm10) and the suggested species boundary of 95-96%, a new species Nitrosomonas supralitoralis sp. nov. is proposed. The type strain of Nitrosomonas supralitoralis is APG5 (= NCIMB 14870 = ATCC TSD-116).
一种β变形菌化能自养氨氧化细菌，被命名为 APG5，是从美国华盛顿州埃德蒙兹市海滩的潮间带沙滩中分离出来的。该菌在 10-35°C（最佳温度为 30°C）、pH5-9（最佳 pH8）和氨浓度高达 100mM（最佳浓度为 1-30mM NHCl）的条件下可以生长。该菌株在淡水培养基中生长最佳，但可耐受高达 400mM 的 NaCl。它与“尿素亚硝化单胞菌”（16S rRNA 和 amoA 序列的同源性分别为 96.7%和 92.4%）最为密切相关。APG5 草案基因组的 375-Mbp 仅包含一个 rRNA 操纵子和所有必需的 tRNA 基因，与第 6 氮单胞菌群中参考菌株的先前报道基因组相比，其 G+C 含量最低（43.5%）。根据与最接近的亲缘种（“尿素亚硝化单胞菌”Nm10）的平均核苷酸同一性为 82%，以及建议的物种边界为 95-96%，提议建立一个新的物种：Nitrosomonas supralitoralis sp. nov.。Nitrosomonas supralitoralis 的模式菌株为 APG5（=NCIMB 14870=ATCC TSD-116）。
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