Sperling L C, Winton G B
Dermatology Service, Walter Reed Army Medical Center.
J Dermatol Surg Oncol. 1990 Dec;16(12):1127-33. doi: 10.1111/j.1524-4725.1990.tb00024.x.
Twenty-five biopsy specimens from the balding frontal scalp of five patients were studied. Processing included vertical and transverse (horizontal) sectioning, toluidine blue and hematoxylin and eosin staining, and fixed and frozen sectioning. Findings included decreased hair density, increased numbers of vellus hairs, and alteration of the normal follicular architecture. Transverse frozen sectioning with toluidine blue staining appeared to be a rapid and reliable tool for studying androgenic alopecia.
对5名患者秃发的额部头皮的25份活检标本进行了研究。处理过程包括垂直和横向（水平）切片、甲苯胺蓝以及苏木精和伊红染色，以及固定和冷冻切片。研究结果包括头发密度降低、毳毛数量增加以及正常毛囊结构改变。甲苯胺蓝染色的横向冷冻切片似乎是研究雄激素性脱发的一种快速且可靠的工具。
