Rehfeld J F, van Solinge W W, Tos M, Thomsen J
Department of Clinical Chemistry, Righospitalet, Copenhagen, Denmark.
Brain Res. 1990 Oct 22;530(2):235-8. doi: 10.1016/0006-8993(90)91288-r.
Using sequence-specific radioimmunoassays the occurrence of 4 neuropeptides (cholecystokinin (CCK), gastrin, somatostatin, substance P and some of their precursors) was examined in 19 human acoustic nerve tumors and corresponding normal tissue. The normal acoustic nerve tissue obtained by autopsy contained traces of CCK, somatostatin, and substance P (less than or equal to 0.5 pmol/g), but neither proCCK, gastrin nor progastrin. In contrast 8 tumors expressed proCCK (range 0.2-4.5 pmol/g), 3 tumors gastrin (0.1-7.3 pmol/g) and 14 tumors progastrin (0.6-2.2 pmol/g). Traces of somatostatin were present in two and substance P in one tumor. The results show that acoustic neuromas often express the homologous CCK and gastrin genes, but process their propeptides poorly to transmitter-active peptides. The tumor synthesis of gastrin/progastrin contrasts to the rare, sporadic expression of the gastrin gene in normal nerve tissue.
运用序列特异性放射免疫分析法，检测了19例人类听神经瘤及相应正常组织中4种神经肽（胆囊收缩素（CCK）、胃泌素、生长抑素、P物质及其一些前体）的存在情况。通过尸检获得的正常听神经组织含有微量的CCK、生长抑素和P物质（小于或等于0.5 pmol/g），但既没有CCK原、胃泌素也没有胃泌素原。相比之下，8例肿瘤表达CCK原（范围为0.2 - 4.5 pmol/g），3例肿瘤表达胃泌素（0.1 - 7.3 pmol/g），14例肿瘤表达胃泌素原（0.6 - 2.2 pmol/g）。有2例肿瘤存在微量生长抑素，1例肿瘤存在P物质。结果表明，听神经瘤常表达同源的CCK和胃泌素基因，但将其前体肽加工成具有递质活性的肽的能力较差。胃泌素/胃泌素原的肿瘤合成与胃泌素基因在正常神经组织中罕见的散在表达形成对比。
