Rehfeld J F, Bardram L, Hilsted L
Department of Clinical Chemistry, Rigshospitalet, University of Copenhagen, Denmark.
Cancer Res. 1989 Jun 1;49(11):2840-3.
Using a library of radioimmunoassays against essential sequences of human progastrin and procholecystokinin, we have examined the occurrence of gastrin, cholecystokinin, and their precursors in bronchogenic adenocarcinomas, large-cell, small-cell, and squamous-cell carcinomas (n = 17). Progastrin and some of its bioactive (i.e., alpha-carboxyamidated) products were present in all tumors, irrespective of histological classification. The concentration of progastrin varied from 0.2 to 21.9 pmol/g tissue; glycine-extended intermediates constituted less than 0.1 to 0.5 pmol/g; and bioactive, carboxyamidated gastrin ranged from less than 0.1 to 6.1 pmol/g. Chromatography showed that the bioactive gastrins were exclusively gastrin-17 peptides, half of which were tyrosine O-sulfated. Neither procholecystokinin nor its processing products were found in the tumor extracts. Six samples of nonneoplastic human lung tissue contained traces of progastrin (range, less than 0.1-0.8 pmol/g), but neither bioactive gastrins nor any cholecystokinin. The results show that the gastrin gene is expressed in all classes of bronchogenic carcinomas. Due to incomplete posttranslational processing measurement of progastrin may be necessary to detect such expression.
利用针对人胃泌素原和胆囊收缩素原基本序列的放射免疫分析文库，我们检测了支气管源性腺癌、大细胞癌、小细胞癌和鳞状细胞癌（n = 17）中胃泌素、胆囊收缩素及其前体的存在情况。无论组织学分类如何，所有肿瘤中均存在胃泌素原及其一些生物活性（即α-羧酰胺化）产物。胃泌素原的浓度在0.2至21.9 pmol/g组织之间；甘氨酸延伸中间体的含量低于0.1至0.5 pmol/g；生物活性的羧酰胺化胃泌素含量在低于0.1至6.1 pmol/g之间。色谱分析表明，生物活性胃泌素均为胃泌素-17肽，其中一半为酪氨酸O-硫酸化肽。在肿瘤提取物中未发现胆囊收缩素原及其加工产物。六份非肿瘤性人肺组织样本中含有微量胃泌素原（范围为低于0.1 - 0.8 pmol/g），但既没有生物活性胃泌素也没有任何胆囊收缩素。结果表明，胃泌素基因在所有类型的支气管源性癌中均有表达。由于翻译后加工不完全，可能需要检测胃泌素原来检测这种表达。
