Yoshida Mutsumi, Babensee Julia E
Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, 313 Ferst Drive, Atlanta, GA 30332, USA.
J Biomater Sci Polym Ed. 2006;17(8):893-907. doi: 10.1163/156856206777996844.
The ability of immature dendritic cells (iDCs) derived from human peripheral blood mononuclear cells to phagocytose poly(lactic-co-glycolic acid) (PLGA) microparticles (MPs) as compared to polystyrene MPs and the molecular aspects of this phagocytosis were investigated. Treating iDCs with PLGA or polystyrene fluorospheres of approximately 3 microm in diameter resulted in the internalization of the particles as evidenced by confocal laser scanning micrographs. This uptake of fluorospheres by DCs was decreased by pretreatment of cells with cytochalasin D or by incubation with the fluorospheres at 4 degrees C, and was sensitive to EDTA and trypsin pretreatments in a dose-dependent manner. In agreement with our previous studies, treatment of iDCs with PLGA MPs, but not with polystyrene MPs, led to DC maturation, as measured by increase in release of the autocrine maturation cytokine, tumor necrosis factor-alpha, which was dependent on ratio of PLGA MPs to DCs. Taken together, this work begins to address the role of phagocytosis on PLGA MP-induced DC maturation and the molecular mechanisms involved.
研究了源自人外周血单核细胞的未成熟树突状细胞（iDCs）吞噬聚乳酸-乙醇酸共聚物（PLGA）微粒（MPs）的能力，并与聚苯乙烯MPs进行了比较，同时研究了这种吞噬作用的分子机制。用直径约3微米的PLGA或聚苯乙烯荧光球处理iDCs，共聚焦激光扫描显微镜图像显示这些颗粒被内化。细胞松弛素D预处理细胞或在4℃下与荧光球孵育会降低DCs对荧光球的摄取，并且对EDTA和胰蛋白酶预处理呈剂量依赖性敏感。与我们之前的研究一致，用PLGA MPs而非聚苯乙烯 MPs处理iDCs会导致DC成熟，这通过自分泌成熟细胞因子肿瘤坏死因子-α的释放增加来衡量，其依赖于PLGA MPs与DCs的比例。综上所述，这项工作开始探讨吞噬作用在PLGA MP诱导的DC成熟中的作用以及相关的分子机制。
