Martínez E, de Vera N, Artigas F
Life Sci. 1991;48(1):77-84. doi: 10.1016/0024-3205(91)90427-d.
The polyamines putrescine (PUT), spermidine (SD) and spermine (SM) have been studied in rat brain after treatment with several convulsant agents. Kainic acid (10 mg/kg), picrotoxinin (1.5 mg/kg), pentylenetetrazol (60 mg/kg) and lindane (gamma-hexachlorocyclohexane) (60 mg/kg) were given to male Wistar rats. Twenty-four hours later, the animals were sacrificed and their brains removed. Cortical polyamines were analyzed by HPLC with fluorimetric detection of their respective dansyl derivatives, using 1,6-diaminohexane as internal standard for the measurements. Polyamine levels are not affected by short periods of time (30 min) of brain exposure to room temperature before freezing the samples, as compared to a quick procedure (less than 40 s from animal death). Kainic acid induced a 14-fold increase of cortical PUT with respect to control values, leaving unchanged the other polyamines. Lindane also increased cortical PUT (4-fold) without affecting SM or SD. Neither picrotoxinin, nor pentylenetetrazol groups were different from controls for any of the polyamines assayed. The results are discussed in relation to the possible mechanism of action of these convulsant agents and the role of the polyamines in cell injury.
在用几种惊厥剂处理大鼠脑之后，对多胺腐胺（PUT）、亚精胺（SD）和精胺（SM）进行了研究。给雄性Wistar大鼠注射 kainic 酸（10 mg/kg）、印防己毒素（1.5 mg/kg）、戊四氮（60 mg/kg）和林丹（γ-六氯环己烷）（60 mg/kg）。24小时后，处死动物并取出其大脑。使用1,6-二氨基己烷作为测量的内标，通过高效液相色谱法（HPLC）对皮质多胺进行分析，并对其各自的丹磺酰衍生物进行荧光检测。与快速程序（动物死亡后少于40秒）相比，在冷冻样品之前，大脑短时间（30分钟）暴露于室温不会影响多胺水平。与对照值相比，kainic 酸使皮质PUT增加了14倍，而其他多胺保持不变。林丹也使皮质PUT增加（4倍），而不影响SM或SD。对于所检测的任何多胺，印防己毒素组和戊四氮组与对照组均无差异。结合这些惊厥剂可能的作用机制以及多胺在细胞损伤中的作用对结果进行了讨论。
