Tan Bo, Bradshaw Heather B, Rimmerman Neta, Srinivasan Harini, Yu Y William, Krey Jocelyn F, Monn M Francesca, Chen Jay Shih-Chieh, Hu Sherry Shu-Jung, Pickens Sarah R, Walker J Michael
Gill Center for Biomolecular Science, Indiana University, Bloomington, IN, USA.
AAPS J. 2006 Jul 14;8(3):E461-5. doi: 10.1208/aapsj080354.
The discovery of endogenous fatty acyl amides such as N-arachidonoyl ethanolamide (anandamide), N-oleoyl ethanolamide (OEA), and N-arachidonoyl dopamine (NADA) as important signaling molecules in the central and peripheral nervous system has led us to pursue other unidentified signaling molecules. Until recently, technical challenges, particularly those associated with lipid purification and chemical analysis, have hindered the identification of low abundance signaling lipids. Improvements in chromatography and mass spectrometry (MS) such as miniaturization of high-performance liquid chromatography components, hybridization of multistage mass spectrometers and time-of-flight technology, the development of electrospray ionization (ESI) and of information-dependent acquisition, now permit rapid identification of novel, low abundance, signaling lipids.
内源性脂肪酰基酰胺类物质的发现，如N-花生四烯酸乙醇酰胺（花生四烯乙醇胺）、N-油酰乙醇酰胺（OEA）和N-花生四烯酸多巴胺（NADA），它们作为中枢和外周神经系统中的重要信号分子，促使我们去寻找其他尚未确定的信号分子。直到最近，技术上的挑战，尤其是那些与脂质纯化和化学分析相关的挑战，阻碍了低丰度信号脂质的鉴定。色谱法和质谱（MS）的改进，如高效液相色谱组件的小型化、多级质谱仪与飞行时间技术的结合、电喷雾电离（ESI）的发展以及信息依赖型采集技术的发展，现在使得快速鉴定新型、低丰度的信号脂质成为可能。
