Banchereau J, de Paoli P, Vallé A, Garcia E, Rousset F
Laboratory for Immunological Research, Dardilly, France.
Science. 1991 Jan 4;251(4989):70-2. doi: 10.1126/science.1702555.
CD40 is a 45- to 50-kilodalton transmembrane glycoprotein expressed on B lymphocytes, epithelial cells, and some carcinoma cell lines. Human resting B lymphocytes entered a state of sustained proliferation when incubated with both the mouse fibroblastic Ltk- cell line that had been transfected with the human Fc receptor (Fc gamma RII/CDw32) and monoclonal antibodies to CD40. In combination with interleukin-4, factor-dependent long-term normal human B cell lines were generated that were consistently negative for Epstein-Barr viral infection. Thus, cross-linking of CD40 is likely to represent an important phenomenon in the clonal expansion of B cells.
CD40是一种45至50千道尔顿的跨膜糖蛋白，表达于B淋巴细胞、上皮细胞及某些癌细胞系。当人静止B淋巴细胞与转染了人Fc受体（FcγRII/CDw32）的小鼠成纤维细胞Ltk-细胞系以及抗CD40单克隆抗体共同孵育时，会进入持续增殖状态。与白细胞介素-4联合使用时，可产生对爱泼斯坦-巴尔病毒感染始终呈阴性的因子依赖性长期正常人B细胞系。因此，CD40的交联可能是B细胞克隆扩增中的一个重要现象。
