Burlinson E L, Pringle H M, Ozanne B W, Cushley W
Division of Biochemistry and Molecular Biology, University of Glasgow, Scotland, UK.
Immunol Lett. 1995 Feb;45(1-2):93-8. doi: 10.1016/0165-2478(94)00232-g.
In this report, the effect of ligation of a number of B-cell surface molecules upon expression of CD25, the 55-kDa inducible component of the IL-2 receptor complex found on T and B lymphocytes, is reported. IL-4 is the only cytokine apparently capable of promoting CD25 expression in human high-density quiescent tonsillar B cells; neither IL-10 nor IL-13 could induce CD25 expression. Cross-linking of the antigen receptors or CD40 with antibody elicited CD25 expression in a dose-dependent manner. Stimulation with anti-CD40 promoted CD25 expression in approximately 25% of B cells, while anti-Ig caused 80% or more of cells to become CD25+. In experiments where the stimuli were used in combination, some additive effects upon CD25 expression were noted, but no obvious synergistic effects could be detected.
在本报告中，报道了多种B细胞表面分子的结扎对CD25表达的影响，CD25是在T和B淋巴细胞上发现的IL-2受体复合物的55 kDa诱导成分。IL-4是唯一显然能够促进人高密度静止扁桃体B细胞中CD25表达的细胞因子；IL-10和IL-13均不能诱导CD25表达。用抗体交联抗原受体或CD40以剂量依赖性方式引发CD25表达。用抗CD40刺激可促进约25%的B细胞中CD25表达，而抗Ig则使80%或更多的细胞变为CD25阳性。在联合使用刺激物的实验中，注意到对CD25表达有一些相加作用，但未检测到明显的协同作用。
