Cheong H S, Chang J S, Park J M, Byun S M
Immunology Laboratory, Mogam Biotechnology Research Institute, Kyonggi-Do, Korea.
Biochem Biophys Res Commun. 1990 Dec 31;173(3):795-800. doi: 10.1016/s0006-291x(05)80857-5.
For the enhancement of antibody binding affinity, a bispecific antibody against two different epitopes in human chorionic gonadotropin hormone, one is in alpha-subunit and the other is in beta-subunit, was prepared by chemical recombination using 5,5'-dithiobis(2-nitrobenzoic acid). The epitopes recognized by antibodies were investigated by competitive radioimmunoassay, two-site sandwich radioimmunoassay and additivity assay and a proper epitope pair was chosen for preparation of the bispecific antibody. This bispecific antibody has dual specificity and as much as 17.2-fold higher affinity than that of monoclonal antibody with higher affinity by dual antigen binding radioimmunoassay and Scatchard plot analysis.
