用于人绒毛膜促性腺激素的固相竞争性和夹心型红细胞免疫测定法。

Solid-phase competitive and sandwich-type erythro-immunoassays for human chorionic gonadotropin.

作者信息

Gupta S K, Guesdon J L, Avrameas S, Talwar G P

出版信息

J Immunol Methods. 1985 Jun 25;80(2):177-87. doi: 10.1016/0022-1759(85)90019-5.

Abstract

A simple '1-step' competitive erythro-immunoassay for human chorionic gonadotropin (hCG) employing V-shaped well microtitration plates coated with monoclonal anti-beta-hCG antibody has been described. hCG of the test sample competes with the antigen-coupled sheep erythrocytes for binding to the antibody on the solid surface. The assay is able to detect up to 31.25 ng hCG/ml. A higher sensitivity enabling detection up to 0.25 ng hCG/ml is attained by the sandwich erythro-immunoassay using a chimera antibody prepared by coupling monoclonal anti-alpha-hCG antibody to an affinity-purified polyclonal antibody specific for sheep erythrocytes. This assay is amenable to the qualitative as well as quantitative use as described. The urinary components do not interfere in the assay. Results obtained by this assay on 47 human urine samples correlated well with the values obtained by '2-step' sandwich enzyme immunoassay and radioimmunoassay.

摘要

本文描述了一种简单的“一步法”竞争性红细胞免疫测定法，用于检测人绒毛膜促性腺激素（hCG），该方法采用包被有抗β-hCG单克隆抗体的V型孔微量滴定板。测试样品中的hCG与抗原偶联的绵羊红细胞竞争结合固相表面的抗体。该测定法能够检测低至31.25 ng hCG/ml的hCG。通过夹心红细胞免疫测定法可实现更高的灵敏度，该方法使用通过将抗α-hCG单克隆抗体与对绵羊红细胞具有特异性的亲和纯化多克隆抗体偶联而制备的嵌合抗体，能够检测低至0.25 ng hCG/ml的hCG。如前所述，该测定法适用于定性和定量检测。尿液成分不干扰该测定法。通过该测定法对47份人类尿液样本获得的结果与通过“两步法”夹心酶免疫测定法和放射免疫测定法获得的值具有良好的相关性。