Lin A, Yan W H, Dai M Z, Chen X J, Li B L, Chen B G, Fan L A
Medical Research Center, Taizhou Hospital, Wenzhou Medical College, Linhai, Zhejiang, People's Republic of China.
Tissue Antigens. 2006 Oct;68(4):311-6. doi: 10.1111/j.1399-0039.2006.00667.x.
Pre-eclampsia is a multisystem disorder of pregnancy and remains the leading cause of both maternal and fetal morbidity and mortality in many countries. Despite extensive studies, the underlying mechanisms still remain unknown. Besides its restricted expression in the tissues of placenta and its function in regulating immune suppression and in ensuring successful invasion of placental tissues into maternal deciduas, it has been postulated that HLA-G may play a role in modulation of immune tolerance at the fetal-maternal interface. Aberrant HLA-G expression may result in pregnancy disorders that are associated with poor invasion of extravillous cytotrophoblast into maternal spiral arteries, such as pre-eclampsia. Studies have shown that pre-eclampsia is largely under genetic control, but genetic mechanisms underlying the disorder have yet to be determined. In the current study, we focus on the potential role of HLA-G polymorphism in the pathogenesis of pre-eclampsia. Samples were obtained from Chinese Han primiparous women with pre-eclampsia and irrelative normal women, and case-matched placentas were genotyped for the HLA-G polymorphism in the exons 2, 3, and 4, and the 14-base-pair (bp) insertion/deletion polymorphism in the 3'-untranslated region of exon 8 was analyzed separately. The frequency of HLA-G polymorphism in these samples was not significantly different from those of normal controls, indicating that maternal HLA-G polymorphism is not associated with the risk for pre-eclampsia in this Chinese Han population. However, the maternal 14-bp insertion/deletion polymorphism is ethnically different.
子痫前期是一种妊娠多系统疾病，在许多国家仍然是孕产妇和胎儿发病及死亡的主要原因。尽管进行了广泛研究，其潜在机制仍然不明。除了在胎盘组织中有限表达及其在调节免疫抑制和确保胎盘组织成功侵入母体蜕膜中的作用外，据推测HLA - G可能在母胎界面免疫耐受调节中发挥作用。HLA - G表达异常可能导致与绒毛外细胞滋养层侵入母体螺旋动脉不良相关的妊娠疾病，如子痫前期。研究表明，子痫前期在很大程度上受遗传控制，但该疾病的遗传机制尚未确定。在本研究中，我们关注HLA - G多态性在子痫前期发病机制中的潜在作用。样本取自患有子痫前期的中国汉族初产妇和不相关的正常女性，并对病例匹配的胎盘进行外显子2、3和4中HLA - G多态性的基因分型，同时分别分析外显子8的3'非翻译区中14个碱基对（bp）的插入/缺失多态性。这些样本中HLA - G多态性的频率与正常对照组无显著差异，表明在这个中国汉族人群中，母体HLA - G多态性与子痫前期风险无关。然而，母体14 bp插入/缺失多态性存在种族差异。
