Husain S M, Shou Y, Sorrentino B P, Handgretinger R
Division of Stem Cell Transplantation, Department of Hematology-Oncology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA.
Tissue Antigens. 2006 Oct;68(4):317-24. doi: 10.1111/j.1399-0039.2006.00679.x.
Human prominin-1 (CD133 or AC133) is an important cell surface marker used to isolate primitive hematopoietic stem cells. The commercially available antibody to human prominin-1 does not recognize rhesus prominin-1. Therefore, we isolated, cloned and characterized the complementary DNA (cDNA) of rhesus prominin-1 gene and determined its coding potential. Following the nomenclature of prominin family of genes, we named this cDNA as rhesus prominin-1.s1. The amino acid sequence data of the putative rhesus prominin-1.s1 could be used in designing antigenic peptides to raise antibodies for use in isolation of pure populations of rhesus prominin-1(+) hematopoietic cells. To the best of our knowledge, there has been no previously published report about the isolation of a prominin-1 cDNA from rhesus monkey (Macaca mulatta).
人促红细胞生成素-1（CD133或AC133）是一种用于分离原始造血干细胞的重要细胞表面标志物。市售的抗人促红细胞生成素-1抗体不能识别恒河猴促红细胞生成素-1。因此，我们分离、克隆并鉴定了恒河猴促红细胞生成素-1基因的互补DNA（cDNA），并确定了其编码潜能。按照促红细胞生成素基因家族的命名法，我们将该cDNA命名为恒河猴促红细胞生成素-1.s1。推测的恒河猴促红细胞生成素-1.s1的氨基酸序列数据可用于设计抗原肽，以制备用于分离纯恒河猴促红细胞生成素-1（+）造血细胞群体的抗体。据我们所知，此前尚无关于从恒河猴（猕猴）中分离促红细胞生成素-1 cDNA的报道。
