Fargeas C A, Huttner W B, Corbeil D
Tissue Engineering Laboratories, BIOTEC, Technical University of Dresden, Dresden, Germany.
Tissue Antigens. 2007 Jun;69(6):602-6. doi: 10.1111/j.1399-0039.2007.00825.x.
Prominin-1 (CD133), a pentaspan membrane glycoprotein that constitutes an important cell surface marker of various, either normal or cancerous, stem cell populations is widely used to isolate or characterize such cells in different systems. Occurring throughout the metazoan evolution with a remarkably conserved genomic organization, it may be expressed as different splice variants with distinctive characteristics. A rational nomenclature has been proposed earlier for their consistent designation across species. Although generally accepted, it seems to be misunderstood in view of the recent report of novel prominin-1 complementary DNAs in rhesus monkey and humans with improper naming. As this may lead to confusion, we have reexamined the genomic organization of prominin-1 in various primates to provide an update that should further clarify the rationale of the nomenclature for prominin-1 gene products. This report comprises (i) the determination of the genomic organization of prominin-1 gene in two non-human primates, i.e. Macaca mulatta and Pan troglodytes, commonly used in research, (ii) the mapping of a new exon that creates an alternative cytoplasmic C-terminal end of prominin-1, (iii) the identification of various potential PDZ-binding domains generated by alternative cytoplasmic C-terminal tails, suggesting that different prominin-1 splice variants might interact with distinct protein partners, and (iv) a summing up of the different prominin-1 splice variants.
Prominin-1（CD133）是一种五跨膜糖蛋白，是各种正常或癌性干细胞群体的重要细胞表面标志物，在不同系统中广泛用于分离或鉴定此类细胞。它在整个后生动物进化过程中都有出现，基因组组织非常保守，可能会以具有独特特征的不同剪接变体形式表达。此前已提出一种合理的命名法，以便在不同物种间对其进行一致的命名。尽管该命名法已被普遍接受，但鉴于最近在恒河猴和人类中发现了命名不当的新型prominin-1互补DNA的报道，它似乎被误解了。由于这可能会导致混淆，我们重新审视了各种灵长类动物中prominin-1的基因组组织，以提供一个更新内容，进一步阐明prominin-1基因产物命名法的基本原理。本报告包括：（i）确定了两种常用于研究的非人类灵长类动物，即猕猴和黑猩猩中prominin-1基因的基因组组织；（ii）绘制了一个新外显子的图谱，该外显子形成了prominin-1的一个替代性胞质C末端；（iii）鉴定了由替代性胞质C末端尾巴产生的各种潜在PDZ结合结构域，这表明不同的prominin-1剪接变体可能与不同的蛋白质伙伴相互作用；（iv）总结了不同的prominin-1剪接变体。
