Andreadis Dimitrios, Lorenzini Guido, Drakoulakos Drakoulis, Belazi Maria, Mihailidou Euaggelia, Velkos Georgios, Mourellou-Tsatsou Olga, Antoniades Demetrios
Department of Oral Medicine and Maxillofacial Pathology, School of Dentistry, Aristotle University of Thessaloniki, Greece.
Eur J Oral Sci. 2006 Oct;114(5):374-80. doi: 10.1111/j.1600-0722.2006.00394.x.
Although there is much literature on the detection of pemphigus and pemphigoid autoantibodies by enzyme-linked immunosorbent assay (ELISA) in serum, nothing is known about their presence in saliva. The aim of this study was to evaluate the salivary levels of these autoantibodies in pemphigus and pemphigoid patients. Autoantibodies against desmoglein3, desmoglein1, and BP180 were assayed, by ELISA, in serum and saliva samples of patients and healthy controls. The titres of autoantibodies against Dsg1/3 found in both serum and saliva of pemphigus patients showed a statistically significant correlation, suggesting that saliva may be a useful biological material for diagnostic purposes, in monitoring disease activity, as well as for the early detection of relapses. By contrast, the titres of autoantibodies against BP180 in the serum and saliva of bullous pemphigoid patients were not statistically related, and further study of the usefulness of the BP180 ELISA for saliva in this disease is needed. In addition, based on our results, the BP180 ELISA with a recombinant NC16a epitope failed to detect the autoantibodies against BP180 in the serum and saliva of mucous membrane pemphigoid patients.
虽然有许多文献报道了通过酶联免疫吸附测定（ELISA）检测血清中的天疱疮和类天疱疮自身抗体，但对于它们在唾液中的存在情况却一无所知。本研究的目的是评估天疱疮和类天疱疮患者唾液中这些自身抗体的水平。通过ELISA检测患者和健康对照者血清及唾液样本中针对桥粒芯糖蛋白3、桥粒芯糖蛋白1和BP180的自身抗体。天疱疮患者血清和唾液中针对桥粒芯糖蛋白1/3的自身抗体滴度显示出统计学上的显著相关性，这表明唾液可能是用于诊断目的、监测疾病活动以及早期检测复发的有用生物材料。相比之下，大疱性类天疱疮患者血清和唾液中针对BP180的自身抗体滴度无统计学相关性，因此需要进一步研究BP180 ELISA在该疾病唾液检测中的实用性。此外，根据我们的结果，使用重组NC16a表位的BP180 ELISA未能检测出黏膜类天疱疮患者血清和唾液中针对BP180的自身抗体。
