Jiang G W, Chen X M, Li L S
Department of Nephrology, Chinese Great Wall Hospital, Beijing.
Chin Med J (Engl). 1990 Oct;103(10):800-4.
In vitro study of rat glomerular mesangial cell growth was carried out to investigate the pattern of serum renotropic stimulation in 5/6 nephrectomized (5/6NT) rats. Incorporation rates of 3H-TdR, 3H-UR and 3H-Leu into mesangial cells were used as parameters to measure serum renotropic stimulation. Renotropin activity increased immediately after 5/6NT, reaching peak at 48 h, and was succeeded by a rapid decline at 96 h postoperatively. A second rise occurred on the 7th day, followed by gradual decrement. It seemed evident that the serum obtained 48 h after 5/6NT surgery was capable of stimulating the uptake of 3H-TdR, 3H-UR and 3H-Leu by the mesangial cells (MCs). It may be concluded that renotropin promotes DNA, RNA and protein synthesis of MCs. The prolonged and constant effect of serum renotropin on MCs might be one of the important mechanisms in progressive sclerosis of the remaining kidney after subtotal nephrectomy.
进行大鼠肾小球系膜细胞生长的体外研究，以探讨5/6肾切除（5/6NT）大鼠血清促肾生长刺激的模式。将3H-TdR、3H-UR和3H-Leu掺入系膜细胞的速率用作测量血清促肾生长刺激的参数。促肾生长素活性在5/6NT后立即增加，在48小时达到峰值，术后96小时迅速下降。第二次升高发生在第7天，随后逐渐下降。很明显，5/6NT手术后48小时获得的血清能够刺激系膜细胞（MCs）摄取3H-TdR、3H-UR和3H-Leu。可以得出结论，促肾生长素促进MCs的DNA、RNA和蛋白质合成。血清促肾生长素对MCs的长期持续作用可能是肾次全切除术后残余肾脏进行性硬化的重要机制之一。
